Nanoassay development

荧光光谱本质上是一种非常敏感的技术；它已经形成了 这是大多数非放射性实时检测的基础，如聚合酶链式反应。我们的实验室已经与 以前的研究员(现在是生物技术行业的人)开发像“CataCave”这样的PCR替代品 探索SNPs，导致出版。 我们还研究了DNA组分与多层金属纳米颗粒的偶联 对于更快的聚合酶链式反应分析和使用FCS(见MPM报告)来量化非常紧密 蛋白质-蛋白质和蛋白质-DNA在亚微升液滴中的结合(分析物 以亚毫微摩尔量存在)。我们还在研究结构转型 使用两种MPM-FCS，G-四链和线型之间的DNA含量相似 和时间分辨荧光工具，目标是开发非常灵敏的G-Quad 检测化验。 我们将时间分辨检测与翻译迁移率(FCS)相结合，以帮助识别用于分析的“自由”和“绑定”签名。

Fluorescence Spectroscopy is inherently a very sensitive technique; it already forms the basis of most non-radioactive real time assays like PCR. Our lab has collaborated with former fellows (now in biotech industry) to develop alternatives to PCR like "CataCleave" probes for SNPs, leading to publication. We also have studied the coupling of DNA components to multilayer metal nanoparticles for much faster PCR analysis and the use of FCS (see MPM report) to quantify very tight protein-protein and protein-DNA binding in sub-microliter drops (analytes are present in sub-femtomole amounts). We are also studying the structural transitions of similar amounts of DNA between G-quadruplexed and linear forms, using both MPM-FCS and Time-Resolved Fluorescence tools, with the goal of developing very sensitive 'G-quad' detection assays. We have combined time-resolved detection with translational mobility (FCS) to help identify "free" and "bound" signatures for assay.