Nanoassay development

荧光光谱本质上是一种非常敏感的技术；它已经形成了 这是大多数非放射性实时检测的基础，如聚合酶链式反应。我们的实验室之前曾与 以前的研究员(现在是生物技术行业的人)开发像“CataCave”这样的PCR替代品 SNPs的探测。 我们继续研究dna组分与多层金属纳米颗粒的光物理和适当的偶联。 对于更快的聚合酶链式反应分析和使用FCS(见MPM报告)来量化非常紧密 蛋白质-蛋白质和蛋白质-DNA在亚微升液滴中的结合(分析物 以亚毫微摩尔量存在)。我们(今年)暂时停止了MPM-FCS 将时间分辨荧光和时间分辨荧光结合在一起；在前几年，我们将时间分辨荧光检测与翻译迁移率(FCS)结合在一起，以帮助识别用于分析的“自由”和“结合”签名。我们目前正在设计用于DNA/RNA探测的STAQ探针(见纳米技术项目)，类似于瀑布，计划只超分辨紧密结合部位。 最近，我们已经使用TCSPC开始解决设计的适体异质性问题(参见MPM项目和TR项目)。 今年，我们还检查了多肽和DNA激子探针的签名，这些探针除非在工业界的合作下展开或切割，否则会自我抑制。

Fluorescence Spectroscopy is inherently a very sensitive technique; it already forms the basis of most non-radioactive real time assays like PCR. Our lab previously collaborated with former fellows (now in biotech industry) to develop alternatives to PCR like "CataCleave" probes for SNPs. We continue to study the photophysics and proper coupling of DNA components to multilayer metal nanoparticles for much faster PCR analysis and the use of FCS (see MPM report) to quantify very tight protein-protein and protein-DNA binding in sub-microliter drops (analytes are present in sub-femtomole amounts). We temporarily suspended (this year) doing MPM-FCS and Time-Resolved Fluorescence together; in previous years, we had numerically combined time-resolved fluorescence detection with translational mobility (FCS) to help identify "free" and "bound" signatures for assay. We are presently designing STAQ probes (see nanoscopy project) for DNA/RNA probing, analagous to catacleave, in plans to only superresolve tight binding sites. Recently we have used TCSPC to begin untangling designed aptamer heterogeneity questions (see MPM project and TR project). This year, we also examined the signatures for peptide and DNA Excitonic probes that self-quench unless unfolded or cleaved, in collaboration with industry.