Cellular Responses to DNA Damage

我们正在努力建立一个体外系统，用于研究在人类细胞中运行的DNA损伤反应系统对检查点激活的影响。 作为一个模型系统，我们正在研究由DNA错配修复蛋白MutSalpha和MutLalpha介导的检查点激活，以响应几种DNA损伤剂，包括SN 1烷化剂，氟尿嘧啶和顺铂。 靶向特定类型DNA损伤的DNA损伤传感器激活ATR和ATM蛋白激酶。 这反过来又激活了包括Chk 1和Chk 2在内的信号激酶级联，最终导致细胞周期停滞在G2/M边界。 我们正在纯化参与这种损伤反应的重组人类蛋白质，目的是确定潜在的分子机制。 我们也有兴趣在识别关键蛋白质复合物的功能，以许可细胞周期阻滞响应DNA损伤剂的组件。

We are working to establish an in vitro system for the study of checkpoint activation by the DNA damage response systems operating in human cells. As a model system, we are investigating checkpoint activation mediated by the DNA mismatch repair proteins MutSalpha and MutLalpha in response to several DNA damaging agents including SN1 alkylators, fluorouracil, and cisplatin. DNA damage sensors that target specific types of DNA damage activate the ATR and ATM protein kinases. This, in turn, activates a cascade of signaling kinases including Chk1 and Chk2 that ultimately result in cell cycle arrest at the G2/M boundary. We are purifying recombinant human proteins involved in this damage response with the goal of defining the underlying molecular mechanism. We are also interested in identifying components of key protein complexes that function to license cell cycle arrest in response to DNA damaging agents.