Protein Chemistry Core

蛋白质化学核心

• 批准号: 8350140
• 负责人: Deborah Morrison
• 金额: $ 20.56万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8350140
• 关键词: Acetylation; Amino Acid Sequence; Amino Acids; Buffers; CCR; Charge; Communities; Computer software; Digestion; Dimensions; Extramural Activities; Gel; High Pressure Liquid Chromatography; Immunoblotting; Institutes; Intramural Research; Isoelectric Focusing; Label; Laboratories; Liquid substance; Maps; Mass Spectrum Analysis; Methods; Methylation; Modification; Molecular Weight; N-terminal; Peptide Mapping; Peptide Sequence Determination; Peptides; Phase; Phosphoamino Acids; Phosphorylation; Phosphorylation Site; Positioning Attribute; Post-Translational Protein Processing; Process; Protein Analysis; Protein Chemistry; Proteins; Proteomics; Radioactive; Radioactive Sequencing; Research Personnel; Sampling; Serine; Services; Site; Staining method; Stains; Techniques; Technology; Threonine; Two-Dimensional Gel Electrophoresis; Tyrosine; United States National Institutes of Health; comparative; drug modification; interest; pressure; protein aminoacid sequence; two-dimensional

During 2010-2011, the Protein Chemistry Core (PCC) provided services to investigators in the NCI-CCR as well as other NIH institutes. In addition, the PCC collaborated with laboratories in the extramural community. 102 samples were processed for N-terminal peptide sequencing. 65 samples were processed for HPLC peptide analysis. 8 samples were processed for peptide digestion. 52 samples were processed for phosphoamino acid analysis. 45 samples were processed for 2D gel electrophoresis. 42 2D-gels were stained or transferred to immobilon for further analysis.

在2010-2011年期间，蛋白质化学核心（PCC）为NCI-CCR以及其他NIH研究所的研究人员提供服务。此外，PCC与校外社区的实验室合作。对102份样品进行N-末端肽测序。处理65个样品用于HPLC肽分析。处理8个样品用于肽消化。对52份样品进行磷酸氨基酸分析。对45份样品进行2D凝胶电泳。将42个2D-凝胶染色或转移到immobilon用于进一步分析。