Protein Chemistry Core

蛋白质化学核心

• 批准号: 8177740
• 负责人: Deborah Morrison
• 金额: $ 19.09万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8177740
• 关键词: Acetylation; Amino Acids; Buffers; Charge; Communities; Computer software; Core Protein; Digestion; Dimensions; Extramural Activities; Gel; High Pressure Liquid Chromatography; Immunoblotting; Institutes; Intramural Research; Isoelectric Focusing; Label; Laboratories; Liquid substance; Maps; Mass Spectrum Analysis; Methods; Methylation; Modification; Molecular Weight; N-terminal; Peptide Mapping; Peptides; Phase; Phosphoamino Acids; Phosphorylation; Phosphorylation Site; Positioning Attribute; Post-Translational Protein Processing; Process; Protein Analysis; Protein Chemistry; Proteins; Proteomics; Radioactive; Radioactive Sequencing; Research Personnel; Sampling; Serine; Services; Site; Staining method; Stains; Techniques; Technology; Threonine; Two-Dimensional Gel Electrophoresis; Tyrosine; United States National Institutes of Health; comparative; drug modification; interest; pressure; protein aminoacid sequence; two-dimensional

During 2009-2010, the Protein Chemistry Core (PCC) provided services to investigators in the NCI-CCR as well as other NIH institutes. In addition, the PCC collaborated with laboratories in the extramural community. 139 samples were processed for N-terminal peptide sequencing. 85 samples were processed for HPLC peptide analysis. 12 samples were processed for peptide digestion. 64 samples were processed for phosphoamino acid analysis. 71 samples were processed for 2D gel electrophoresis. 71 2D-gels were stained or transferred to immobilon for further analysis.

在2009-2010年期间，蛋白质化学核心（PCC）为NCI-CCR以及其他NIH研究所的研究人员提供服务。此外，PCC与校外社区的实验室合作。对139份样品进行N-末端肽测序。处理85个样品用于HPLC肽分析。处理12个样品用于肽消化。对64份样品进行磷酸氨基酸分析。对71份样品进行二维凝胶电泳。将71个2D-凝胶染色或转移到immobilon用于进一步分析。