ANALYTICAL AND RESOURCE CORE SERVICES

分析和资源核心服务

• 批准号: 8357291
• 负责人: JOHN P CAPITANIO
• 金额: $ 4.79万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357291
• 关键词: Allergens; Antibodies; Antigens; Area; Bioinformatics; Biological Assay; Blood; Blood specimen; Bronchoalveolar Lavage; Buffers; Businesses; California; Candidate Disease Gene; Cell Separation; Cells; Clinical; Clinical Research; Color; Communication; Communities; Consultations; Custom; DNA; Detection; Diagnostic; Endocrine; Epithelial Cells; Evaluation; Experimental Designs; Fibroblasts; Flow Cytometry; Funding; Gene Targeting; Genetic; Genome; Grant; HIV; Health Services Accessibility; Health Status; Histamine; Hormones; IgE; Image; Immunoglobulin G; Immunohistochemistry; Immunology; Immunomodulators; In Vitro; Individual; Infectious Agent; Infectious Disease Immunology; Laboratories; Lasers; Life; Link; Lung; Lung diseases; Lymphoid Tissue; Macaca mulatta; Microspheres; Mitochondrial DNA; Modeling; Molecular Analysis; Monitor; Mucous Membrane; National Center for Research Resources; Organization administrative structures; Pathogen detection; Plasma; Preparation; Primates; Principal Investigator; Process; Proteins; RNA; Reagent; Research; Research Infrastructure; Research Personnel; Research Support; Resources; SIV; Sampling; Scientist; Services; Simian virus 40; Site; Source; Staining method; Stains; Steroids; Structure; Structure of parenchyma of lung; Surrogate Markers; Technical Expertise; Testing; Tissues; Training; United States National Institutes of Health; Viral; animal colony; assay development; cell type; cost; human tissue; instrument; interest; monoamine; nonhuman primate; pathogen; tissue processing; viral DNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Analytical and Resource Core was established in January, 2008 to 1) increase communication and efficiencies among laboratories all of which provide, in one form or another, assay services; 2) provide a larger organizational unit that could buffer fluctuations in the amount of business conducted by each individual laboratory; and 3) facilitate user access to the services, and especially to the resources, offered by CNPRC. The "Analytical" component of the ARC includes service functions associated with previously independent Cores at CNPRC, and the "Resource" component of this Core makes available viral stocks, reagents, tissues, and other resources available at CNPRC. Because many of these resources were developed by the same laboratories that provide assay services, the two functions, services and resources, were combined into a single Core. The structure of the ARC comprises the following seven CNPRC laboratories: Clinical Laboratory provides clinical and research support to internal CNPRC units, as well as to outside investigators using our facility, for monitoring the health status of colony animals for both routine and experimental purposes Endocrine Core Laboratory provides to investigators a service of hormone analysis as well as consultation relating to endocrine research conducted with nonhuman primates. This Core Laboratory also develops and validates steroid, monoamine, and protein hormone assays as needed by investigators. Flow Cytometry Core Laboratory provides access to, and services utilizing, two instruments, a FACS Calibur, which can be used for standard 4-color assays, and a FACS Aria, which has 3 lasers and can be used for multicolor flow cytometric analysis and for live cell sorting. The Laboratory performs the staining, acquisition, and analysis of standard Clinical Laboratory panels, but Principal Investigators can request custom panels. Genetics Core Laboratory provides research support in the following areas: custom DNA extraction and purification, whole genome amplification, SNP, STR and mtDNA assays; bioinformatic analysis including whole genome and target region linkage and association analyses; and candidate gene evaluation. Infectious Disease Immunology Core Laboratory provides the following services: blood and tissue processing (including mucosal tissues), antibody ELISAs against whole SIV, flow cytometric assays for phenotypic and functional characterization of multiple cell types (through the Flow Cytometry Core Laboratory), RNA / DNA isolation (blood and tissues) and analysis for several target genes. In addition, the Luminex multiplex microbead assay for the detection of antibodies and multiple immunomodulators in plasma or culture supernatants is available; this includes use of multiplex panels from commercial sources as well as custom assay development. Pathogen Detection Core Laboratory offers antibody, antigen, virus, DNA, RNA and other diagnostic marker detection for a number of infectious agents including HIV, HVP2 (Herpes B surrogate marker), LCV, RRV, SFV, SIV, SRV, STLV, SV40, TB, RhCMV and other non human primate pathogens. Services are provided to investigators and to the CNPRC colony. Custom testing, training, and consultation are also available. Respiratory Disease Immunology Core Laboratory provides technical expertise and resources to investigators interested in the immunological evaluation of rhesus macaque models for lung disease. Services include assistance with experimental design, allergen characterization and preparation, evaluation of allergen-specific IgE and IgG, and quantitation of histamine. The Core Laboratory also provides technical support for sample processing, including bronchoalveolar lavage, lymphoid tissue, lung tissue, and blood. Flow cytometry and molecular analysis of lung and blood samples are coordinated with the Flow Cytometry Core Laboratory and the Infectious Disease Immunology Core Laboratory. Immunohistochemistry of lung samples is also offered in conjunction with the Computational Imaging Core. In addition to immunological evaluation, the Respiratory Disease Immunology Core Laboratory can provide investigators with primary cell isolates and in vitro culture of rhesus lung epithelial cells and fibroblasts. In addition to these seven Laboratories, the ARC has formal links to one Affiliated Laboratory located off-site. This Laboratory is directed by a CNPRC staff scientist, and while it provides a broad range of services to the research community (including assays on human tissue), it also provides services to nonhuman primate researchers using species-specific reagents. By linking to this Laboratory, we can preclude the need to duplicate existing services and insure good communication between all service units across campus that are offering assay services of one type or another to investigators using nonhuman primates.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。对该分项目和该分项目首席调查员的主要支持可能是由其他来源提供的，包括国家卫生研究院的其他来源。列出的子项目总成本可能代表子项目使用的中心基础设施的估计数量，而不是NCRR拨款提供给子项目或子项目工作人员的直接资金。 分析和资源核心于2008年1月成立，目的是1)加强所有实验室之间的沟通和效率，所有这些实验室都以这样或那样的形式提供分析服务；2)提供一个更大的组织单位，可以缓冲每个实验室开展的业务量的波动；以及3)方便用户访问中国人民解放军提供的服务，特别是资源。ARC的“分析”部分包括与中国石油天然气集团公司以前独立的核心相关的服务职能，而该核心的“资源”部分使中国石油天然气集团公司能够获得病毒库存、试剂、组织和其他资源。由于这些资源中的许多都是由提供化验服务的相同实验室开发的，因此服务和资源这两个功能被合并到一个核心中。 ARC的结构包括中国石油天然气集团公司的以下七个实验室： 临床实验室为CNPRC内部单位以及使用我们设施的外部调查人员提供临床和研究支持，用于日常和实验目的监测群体动物的健康状况 内分泌核心实验室为研究人员提供激素分析服务，以及与非人类灵长类动物内分泌研究相关的咨询。该核心实验室还根据研究人员的需要开发和验证类固醇、单胺和蛋白质激素分析。 流式细胞仪核心实验室提供对两台仪器的访问和服务，一台FACS Calibur可用于标准的四色分析，另一台FACS Aria具有3台激光，可用于多色流式细胞仪分析和活细胞分选。实验室执行标准临床实验室面板的染色、采集和分析，但首席调查员可以要求定制面板。 遗传学核心实验室在以下领域提供研究支持：定制DNA提取和纯化、全基因组扩增、SNP、STR和mtDNA分析；生物信息学分析，包括全基因组和目标区域的连锁和关联分析；以及候选基因评估。 传染病免疫学核心实验室提供以下服务：血液和组织处理(包括粘膜组织)、针对整个SIV的抗体ELISA、多种细胞类型的表型和功能表征的流式细胞仪分析(通过Flow Cytomtics核心实验室)、RNA/DNA分离(血液和组织)以及几个目标基因的分析。此外，Luminex多重微珠检测方法可用于检测血浆或培养上清液中的抗体和多种免疫调节剂；这包括使用来自商业来源的多重试板以及定制的分析开发。 病原体检测核心实验室提供抗体、抗原、病毒、DNA、RNA和其他诊断标记物检测多种感染性病原体，包括HIV、HVP2(疱疹B型替代标记物)、LCV、RRV、SFV、SIV、SRV、STLV、SV40、TB、RhCMV和其他非人类灵长类病原体。向调查人员和中国人民解放军殖民地提供服务。还提供定制测试、培训和咨询服务。 呼吸系统疾病免疫学核心实验室为对恒河猴肺部疾病模型的免疫学评估感兴趣的研究人员提供技术专业知识和资源。服务包括协助实验设计、过敏原表征和准备、评估过敏原特异性IgE和Ig G，以及组胺的定量。核心实验室还为样本处理提供技术支持，包括支气管肺泡灌洗、淋巴组织、肺组织和血液。肺和血液样本的流式细胞术和分子分析与流式细胞术核心实验室和传染病免疫学核心实验室协调。肺样本的免疫组织化学也与计算成像核心一起提供。除了免疫学评估，呼吸系统疾病免疫学核心实验室还可以为研究人员提供原代细胞分离和恒河猴肺上皮细胞和成纤维细胞的体外培养。 除了这七个实验室外，ARC还与位于场外的一个附属实验室建立了正式联系。该实验室由中国人民解放军的一名专职科学家领导，在为研究界提供广泛的服务(包括人体组织的分析)的同时，它也使用物种特有的试剂向非人类灵长类研究人员提供服务。通过链接到该实验室，我们可以避免复制现有服务的需要，并确保校园内向使用非人类灵长类动物的研究人员提供某种类型的分析服务的所有服务单位之间的良好沟通。