CORE B: MOLECULAR EXPRESSION AND TRANSGENICS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 核心B-分子表达和转基因：位于安德森研究大楼104D室(约900平方英尺)新装修的实验室空间。该实验室配备了常规分子和细胞生物学实验的所有主要仪器，包括分光光度计、两台聚合酶链式反应热循环仪、A-80冰柜、两台细胞培养孵化器、两台小型台式离心机和一台电穿孔机等。2008年7月，我们收购了一辆全新的贝顿 Dickinson FACSAria II细胞分选机。Aria有三个激发激光器(真的紫外线、蓝色和红色)和11个荧光探测器，可以比Elite(我们之前使用的，目前仍在使用的)至少快一个数量级的分类速度。我们已经设置了它，并开始使用它来对细胞进行排序。CORE B还有位于安德森111A室的胚胎干细胞培养套间和安德森大楼动物设施内的一个单独的显微注射室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core B- Molecular expression and transgenics: is located in the newly renovated lab space in the Anderson research building room 104D (~900 sq.ft). This lab is fully equipped with all major instruments for routine molecular and cellular biology experiments, including a spectrophotometer, two PCR thermal cyclers, a -80 freezer, two cell culture incubators, two mini desktop centrifuges, and an electroporator, etc. Core B has a Backman Coulter Elite cell sorter equipped with an Autoclone for cell sorting. In July 2008, we acquired a brand new Becton Dickinson FACSAria II cell sorter. The Aria has three lasers for excitation (true UV, blue and red) and 11 fluorescence detectors and can sort at least one order of magnitude faster than the Elite (the one that we used before and is still in service). We have set it up and started using for sorting cells. Core B also has ES cell culture suite located in Anderson room 111A and a separate microinjection room inside of the Animal Facility in the Anderson building.