MOLECULAR BIOLOGY/VIRAL VECTOR CORE

分子生物学/病毒载体核心

• 批准号: 8360465
• 负责人: DAVID J POULSEN
• 金额: $ 10.42万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360465
• 关键词: Axon; Centers of Research Excellence; Cloning; Computer software; Core Facility; Environmental Health; Equipment; Funding; Gene Expression; Genomics; Genotype; Grant; Molecular Biology; National Center for Research Resources; Pathway interactions; Principal Investigator; Production; Research; Research Infrastructure; Resources; Reverse Transcriptase Polymerase Chain Reaction; Source; Techniques; Time; United States National Institutes of Health; Viral Vector; cost; instrument

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Molecular Biology Core Facility (MBCF) was formed during the COBREII funding period from the consolidation of two other cores, the Viral Vector and Microarray cores. It has provided users with equipment and expertise in a wide range of techniques, including gene expression analysis, genotyping, cloning, viral vector production, and basic molecular biology. Instruments and equipment currently available in the facility include: + Agilent (Stratagene) Mx3005p Real-time PCR Instrument + Agilent 2100 Bioanalyzer + Axon GenePix 4000B Microarray Scanner + NanoDrop 1000D Spectrophotometer + MJ Research PTC-100 Thermal Cycler + Eppendorf 5810R Refrigerated Centrifuge + Forma Scientific -80¿C Freezer + Roche Universal ProbeLibrary for RT-PCR + Ariadne Genomics Pathway Studio Software The MBCF has been directed by Dr. David Poulsen, and staffed by Corbin Schwanke. Over the past year funding for the core has been solely provided by the CEHS COBRE grants for the Center for Environmental Health Sciences.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 分子生物学核心设施（MBCF）是在COBREII资助期间由另外两个核心（病毒载体和微阵列核心）合并而成。 它为用户提供了广泛技术领域的设备和专业知识，包括基因表达分析、基因分型、克隆、病毒载体生产和基础分子生物学。 该设施目前可用的仪器和设备包括： + Agilent（Stratagene）Mx3005 p实时PCR仪 + Agilent 2100生物分析仪 + Axon GenePix 4000 B微阵列扫描仪 + NanoDrop 1000 D分光光度计 + MJ Research PTC-100热循环仪 + Eppendorf 5810 R冷藏柜 + Forma Scientific -80 ° C冷冻机 + Roche Universal ProbeLibrary for RT-PCR + Ariadne Genomics Pathway Studio软件 MBCF由大卫·鲍尔森博士指导，由科尔宾·施万克担任工作人员。 在过去的一年中，核心的资金完全由CEHS COBRE为环境健康科学中心提供的赠款提供。