MOLECULAR BIOLOGY/VIRAL VECTOR CORE

分子生物学/病毒载体核心

• 批准号: 7959563
• 负责人: DAVID J POULSEN
• 金额: $ 9.77万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959563
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Core Facility; Environmental Health; Equipment; Funding; Gene Expression; Genes; Genotype; Grant; Institution; Mass Spectrum Analysis; Methods; Molecular Biology; Molecular Biology Techniques; Montana; Nucleic Acids; Production; Proteomics; Reporter; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Services; Source; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Universities; Viral Vector; instrument; overexpression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core Facility was created in 2008 by combining the equipment and expertise from two under-utilized cores at The University of Montana. Utilizing the instruments and staff from the Microarray Core Facility and the Viral Vector Core Facility, this new core covers the entire spectrum of molecular biology techniques and applications. The main services provided by the core are: 1) Gene expression analysis by RT-PCR and microarray, 2) Proteomics analysis by 2D gel electrophoresis and mass spectrometry, 3) Genotyping by a variety of methods, 4) Viral vector production for gene overexpression, gene knockdown, and reporter expression, 5) Assistance with basic molecular biology techniques, such as nucleic acids isolation and PCR.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 分子生物学核心设施创建于2008年，结合了蒙大拿大学两个未充分利用的核心的设备和专业知识。 利用微阵列核心设施和病毒载体核心设施的仪器和工作人员，这个新的核心涵盖了分子生物学技术和应用的整个范围。 该中心提供的主要服务有：1）通过RT-PCR和微阵列进行基因表达分析，2）通过2D凝胶电泳和质谱进行蛋白质组学分析，3）通过各种方法进行基因分型，4）用于基因过表达，基因敲减和报告基因表达的病毒载体生产，5）基本分子生物学技术，如核酸分离和PCR。