MOLECULAR BIOLOGY/VIRAL VECTOR CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 拟议的分子生物学/病毒载体核心的主要目标是为CEHS研究人员和其他人提供最新技术支持：1）通过标准分子生物学方法建立高效、细胞类型特异性和组成型基因表达克隆; 2）使用这些构建体来产生纯化的、高滴度的重组腺相关病毒（腺相关病毒）载体。这些服务将提供一种独特的能力，在体外和体内驱动特定基因在选定的细胞群中的过表达或敲低。分子生物学/病毒载体核心的总体目的是为研究人员提供一套新的工具，使他们能够通过实验提出关键问题，从而产生高质量的出版物和初步数据，以支持RO 1资助申请。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary objective of the proposed molecular biology/viral vector core is to provide state of the art support for CEHS investigators and others to: 1) establish highly efficient, cell type specific and constitutive gene expression clones through standard molecular biology methodologies and; 2) use these constructs to generate purified, high titer recombinant adeno-associated virus (AAV) vectors. These services will provide a unique ability to drive the over expression or knock down of specific genes in selected cell populations both in vitro and in vivo. The overall purpose of the molecular biology/viral vector core is to provide investigators with a set of novel tools that will enable them to experimentally ask critical questions leading to high quality publications and the generation of preliminary data, to support RO1 grant applications.