MOLECULAR BIOLOGY/VIRAL VECTOR CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子生物学核心设施成立于2008年，将蒙大拿大学两个未得到充分利用的核心的设备和专业知识结合在一起。利用微阵列核心设备和病毒载体核心设备的仪器和工作人员，这个新的核心涵盖了分子生物学技术和应用的全部范围。CORE提供的主要服务包括：1)用RT-PCR和微阵列进行基因表达分析；2)用2D凝胶电泳法和质谱仪进行蛋白质组学分析；3)用多种方法进行基因分型；4)生产用于基因过表达、基因敲除和报告表达的病毒载体；5)辅助基本的分子生物学技术，如核酸分离和聚合酶链式反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core Facility was created in 2008 by combining the equipment and expertise from two under-utilized cores at The University of Montana. Utilizing the instruments and staff from the Microarray Core Facility and the Viral Vector Core Facility, this new core covers the entire spectrum of molecular biology techniques and applications. The main services provided by the core are: 1) Gene expression analysis by RT-PCR and microarray, 2) Proteomics analysis by 2D gel electrophoresis and mass spectrometry, 3) Genotyping by a variety of methods, 4) Viral vector production for gene overexpression, gene knockdown, and reporter expression, 5) Assistance with basic molecular biology techniques, such as nucleic acids isolation and PCR.