HIGH RESOLUTION IMAGING OF MYO-INSITOL IN ALZHEMIER?S DISEASE PATHOLOGY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 肌肌醇（MI）是存在于人脑中最丰富的代谢物之一。MI是神经胶质细胞增殖的标志物，在脑中起渗透剂的作用。MI的浓度已被证明在各种脑病理中发生变化。质子磁共振波谱（1HMRS）研究表明，阿尔茨海默病（AD）中MI浓度增加。胶质细胞的增殖/活化与AD的病理改变有关。1HMRS已被广泛用于监测AD中MI的变化，然而，其分辨率较差，并且也不能提供关于具有较高胶质细胞增殖/活化的区域的信息。最近，已经通过利用可交换的微阵列进行了人脑中MI的高分辨率映射。 使用通常称为化学交换饱和转移成像（CEST）的技术检测MI上存在的OH质子。在本研究中，我们首次对转基因AD小鼠（APP/PS1）进行MI CEST，以评估与野生型小鼠相比MI浓度的变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Myo-Inositol (MI) is one of the most abundant metabolites present in the human brain. MI is a marker of glial cells proliferation and functions as an osmolyte in brain. The concentration of MI has been shown to change in various brain pathologies. Proton magnetic resonance spectroscopy (1HMRS) studies have shown increased MI concentration in Alzheimer's disease (AD). Glial cells proliferation/activation is associated with the pathological changes in AD. 1HMRS has been widely used to monitor MI changes in AD, however, it suffers from poor resolution and also does not provide information about the region with the higher glial cells proliferation/activation. Recently, high-resolution mapping of MI in human brain has been performed by exploiting exchangeable OH protons present on MI using a technique commonly known as chemical exchange saturation transfer imaging (CEST). In the current study, for the first time we perform MI CEST on transgenic AD mice (APP/PS1) to evaluate the changes in MI concentration compared to wild type mice.