QUANTIFICATION OF METABOLITES WITH EXCHANGEABLE PROTONS USING SPIN-LOCK MRI

使用自旋锁 MRI 对可交换质子代谢物进行定量

• 批准号: 8361972
• 负责人: FELIKS KOGAN
• 金额: $ 3.84万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361972
• 关键词: Animal Model; Chemicals; Detection; Environment; Exhibits; Funding; Grant; Human; Image; Imaging Techniques; Magnetic Resonance; Magnetic Resonance Imaging; Mediating; Methods; Modeling; National Center for Research Resources; Nuclear; Principal Investigator; Property; Proteins; Protons; Relaxation; Research; Research Infrastructure; Resources; Shapes; Site; Source; System; Techniques; Time; United States National Institutes of Health; Water; Work; base; cost; magnetic field; optical imaging

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Much work has been done recently on imaging techniques that utilize chemical exchange of protons to make MR imaging sensitive to concentrations of proteins and their immediate environments. Spin-lattice relaxation in the rotating frame (T1¿) is an imaging technique which is dependent on the exchange of protons but has yet to be applied to imaging specific metabolites based on their chemical exchange properties. There have been no studies to date using T1¿ for contrast in the chemical exchange of protons. T1¿ Chemical exchange effects vary quadratically with the static magnetic field. Therefore, T1¿ potentially offer higher sensitivity at higher fields in probing exchange mediated interactions in nuclear spin systems. This higher sensitivity of T1¿ MRI may enable detection of endogenous metabolites that possess a proton exchange site at low concentrations (~1 mM). Our hypothesis is that Tip based MRI will be less susceptible to line shape changes and provide higher sensitivity in detecting metabolites with exchangeable protons. The purpose of this subproject is to develop a sensitive and robust method to quantify the concentration of and metabolites exhibiting a chemical exchange of protons with water using T1¿. Study Aims: 1. To develop a method to that utilizes T1¿ MRI techniques that can be used to create image contrast and quantify metabolites with exchangeable protons. 2. To determine the effect that concentration, pH, static magnetic field, B1 field strength and saturation time have on the chemical exchange and subsequent T1¿ contrast 3. To demonstrate that our model can be reliably used to quantify the concentration of metabolites with exchangeable protons in animal models and humans.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 最近已经在成像技术上做了很多工作，该成像技术利用质子的化学交换， 使MR成像对蛋白质浓度及其周围环境敏感。 旋转坐标系中的自旋-晶格弛豫（T1？）是一种成像技术， 质子交换，但尚未应用于成像特定的代谢物基于其化学 交换属性。迄今为止，还没有研究使用T1？作为化学对比剂。 质子交换。化学交换效应随静磁场呈二次方变化。 因此，T1 <$在探测交换介导的高场可能提供更高的灵敏度。 核自旋系统中的相互作用 T1 ~ MRI的这种更高的灵敏度可以检测到 在低浓度（约1 mM）下具有质子交换位点的内源性代谢产物。 我们的假设是，基于头端的MRI将不太容易受到线形变化的影响， 在检测具有可交换质子的代谢物方面具有更高的灵敏度。这样做的目的 一个子项目是开发一种敏感和可靠的方法， 使用T1？显示质子与水的化学交换的代谢物。 研究目的： 1.开发一种利用T1 <$MRI技术的方法，可用于创建图像 对比和量化代谢物与可交换质子。 2.为了确定浓度、pH值、静磁场、B1场强和 饱和时间对化学交换和随后的T1 <$对比度的影响 3.为了证明我们的模型可以可靠地用于量化 在动物模型和人类中具有可交换质子的代谢物。