QUANTIFICATION OF METABOLITES WITH EXCHANGEABLE PROTONS USING SPIN-LOCK MRI

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 最近已经在成像技术上做了很多工作，该成像技术利用质子的化学交换， 使MR成像对蛋白质浓度及其周围环境敏感。 旋转坐标系中的自旋-晶格弛豫（T1？）是一种成像技术， 质子交换，但尚未应用于成像特定的代谢物基于其化学 交换属性。迄今为止，还没有研究使用T1？作为化学对比剂。 质子交换。化学交换效应随静磁场呈二次方变化。 因此，T1 â在探测交换介导的过程中可能在更高的场提供更高的灵敏度 核自旋系统中的相互作用 T1 ~ MRI的这种更高的灵敏度可以检测到 低浓度（~1 mM）下具有质子交换位点的内源性代谢产物。 我们的假设是，基于头端的MRI将不太容易受到线形变化的影响， 在检测具有可交换质子的代谢物方面具有更高的灵敏度。这样做的目的 一个子项目是开发一种敏感和可靠的方法， 使用T1？显示质子与水的化学交换的代谢物。 研究目的： 1.开发一种利用T1 ² MRI技术创建图像的方法 对比和量化代谢物与可交换质子。 2.为了确定浓度、pH值、静磁场、B1场强和 饱和时间对化学交换和随后的T1 <$对比度的影响 3.为了证明我们的模型可以可靠地用于量化 在动物模型和人类中具有可交换质子的代谢物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much work has been done recently on imaging techniques that utilize chemical exchange of protons to make MR imaging sensitive to concentrations of proteins and their immediate environments. Spin-lattice relaxation in the rotating frame (T1¿) is an imaging technique which is dependent on the exchange of protons but has yet to be applied to imaging specific metabolites based on their chemical exchange properties. There have been no studies to date using T1¿ for contrast in the chemical exchange of protons. T1¿ Chemical exchange effects vary quadratically with the static magnetic field. Therefore, T1¿ potentially offer higher sensitivity at higher fields in probing exchange mediated interactions in nuclear spin systems. This higher sensitivity of T1¿ MRI may enable detection of endogenous metabolites that possess a proton exchange site at low concentrations (~1 mM). Our hypothesis is that Tip based MRI will be less susceptible to line shape changes and provide higher sensitivity in detecting metabolites with exchangeable protons. The purpose of this subproject is to develop a sensitive and robust method to quantify the concentration of and metabolites exhibiting a chemical exchange of protons with water using T1¿. Study Aims: 1. To develop a method to that utilizes T1¿ MRI techniques that can be used to create image contrast and quantify metabolites with exchangeable protons. 2. To determine the effect that concentration, pH, static magnetic field, B1 field strength and saturation time have on the chemical exchange and subsequent T1¿ contrast 3. To demonstrate that our model can be reliably used to quantify the concentration of metabolites with exchangeable protons in animal models and humans.