DOES DIDS PROTECT AGAINST ISCHEMIC INSULTS IN CULTURED NEURONS OR ASTROCYTES?

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在培养的神经元或星形胶质细胞中，DDS对缺血损伤有保护作用吗？ 4，4-二异硫氰基二苯二磺酸(DIDS)是一种具有细胞保护作用的化合物，具有多种细胞靶点。以前我们的实验室报道，在缺血损伤(IS)期间，DIDS为海马片提供了90%的细胞保护。然而，最近的观察表明，DDS处理的细胞可能不会存活。为了解决这一矛盾，我们正在使用分子和生化活性分析、电生理学、延时共聚焦显微镜和透射电子显微镜(TEM)来研究DDS对常氧条件下细胞活性的影响，以及对神经元和星形胶质细胞的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DOES DIDS PROVIDE PROTECTION AGAINST ISCHEMIC INSULTS IN CULTURED NEURONS OR ASTROCYTES? 4,4-diisothiocyanatostilbenedisulphonic acid (DIDS) is a putatively cytoprotective compound with numerous cellular targets. Previously our laboratory reported that DIDS provided > 90% cytoprotection in hippocampal slices during ischemic insults (IS). However, recent observations suggest that DIDS-treated cells may not be viable. To address this inconsistency we are examining the effect of DIDS on cellular viability during normoxia and IS in neurons and astrocytes using molecular and biochemical viability assays, electrophysiology, time-lapse confocal microscopy, and transmission electron microscopy (TEM).