DOES DIDS PROTECT AGAINST ISCHEMIC INSULTS IN CULTURED NEURONS OR ASTROCYTES?

是否可以保护培养的神经元或星形胶质细胞免受缺血性损伤？

• 批准号: 8361916
• 负责人: Gabriel G Haddad
• 金额: $ 1.48万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361916
• 关键词: Acids; Address; Astrocytes; Biochemical; Biological Assay; Cells; Confocal Microscopy; Cytoprotection; Electrophysiology (science); Funding; Grant; Hippocampus (Brain); Image Analysis; Laboratories; Molecular; National Center for Research Resources; Neurons; Principal Investigator; Reporting; Research; Research Infrastructure; Resources; Slice; Source; Time; Transmission Electron Microscopy; United States National Institutes of Health; cellular targeting; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DOES DIDS PROVIDE PROTECTION AGAINST ISCHEMIC INSULTS IN CULTURED NEURONS OR ASTROCYTES? 4,4-diisothiocyanatostilbenedisulphonic acid (DIDS) is a putatively cytoprotective compound with numerous cellular targets. Previously our laboratory reported that DIDS provided > 90% cytoprotection in hippocampal slices during ischemic insults (IS). However, recent observations suggest that DIDS-treated cells may not be viable. To address this inconsistency we are examining the effect of DIDS on cellular viability during normoxia and IS in neurons and astrocytes using molecular and biochemical viability assays, electrophysiology, time-lapse confocal microscopy, and transmission electron microscopy (TEM).

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在培养的神经元或星形胶质细胞中，DIDS是否对缺血性损伤提供保护？ 4，4-二异硫氰基芪二磺酸（DIDS）是一种具有多种细胞靶点的嘌呤类细胞保护化合物。此前我们的实验室报告称，DIDS在缺血性损伤（IS）期间对海马切片提供了> 90%的细胞保护。然而，最近的观察表明，DIDS处理的细胞可能无法存活。为了解决这种不一致性，我们正在研究DIDS对细胞活力的影响，在常氧和IS在神经元和星形胶质细胞中使用分子和生化活力测定，电生理学，延时共聚焦显微镜，和透射电子显微镜（TEM）。