MULTIPLEXED ANALYSIS OF INFLUENZA VIRUS TYPE, SUB-TYPE, & RECEPTOR SPECIFICITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 为了便于流感病毒的基础研究和监测，我们发展了一种基于多重微粒的分析方法，用于同时进行基于抗体的毒株分型和亚型鉴定，以及碳水化合物受体结合偏好的表征。基于免疫分析的病毒亚型和受体结合表型测量的结合为追踪禽流感病毒株对人类宿主的适应性提供了一个重要的新工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In order to facilitate the characterization of flu virus for both basic research and surveillance, we have developed a multiplexed microparticle-based assay for simultaneous antibody-based strain typing and sub-typing, as well characterization of carbohydrate receptor binding preference. The combination of immunoassay-based virus sub-typing and receptor binding phenotype measurements provides an important new tool in tracking the adaptation of avian flu strains to human hosts.