VISUALIZATION OF CELLULAR COMPONENTS IN XENOPUS LAEVIS OOCYTES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 小鼠早期卵母细胞是瞬时极性的：三维和超微结构分析。许多无脊椎动物和非哺乳类脊椎动物物种的卵母细胞不仅是不对称的，而且在细胞器、局部RNA和蛋白质的分布上也是极性的，并且卵母细胞极性决定了未来胚胎的模式。Balbiani小体（Bb）位于许多物种卵母细胞的极性，已知在非洲爪蟾中与决定生殖细胞命运的生殖颗粒相关。相反，在哺乳动物中，人们普遍认为胚胎的图案化在植入前不会发生，并且卵母细胞是非极性和对称的。虽然许多哺乳动物的卵母细胞，包括小鼠和人类，含有Bbs，但Bbs的存在与小鼠卵母细胞和卵子极性如何以及是否相关仍然未知。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Mouse early oocytes are transiently polar: three-dimensional and ultrastructural analysis. The oocytes of many invertebrate and non-mammalian vertebrate species are not only asymmetrical but also polar in the distribution of organelles, localized RNAs and proteins, and the oocyte polarity dictates the patterning of the future embryo. Polarly located within the oocytes of many species is the Balbiani body (Bb), which in Xenopus is known to be associated with the germinal granules responsible for the determination of germ cell fate. In contrast, in mammals, it is widely believed that the patterning of the embryo does not occur before implantation, and that oocytes are non-polar and symmetrical. Although the oocytes of many mammals, including mice and humans, contain Bbs, it remains unknown how and if the presence of Bbs relates to mouse oocyte and egg polarity.