SINGAPORE GROUPER IRIDOVIRUS (SGIV)

新加坡石斑鱼虹彩病毒 (SGIV)

• 批准号: 8361140
• 负责人: Jia Wu
• 金额: $ 1.23万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361140
• 关键词: Amphibia; Aquaculture; Area; Caliber; Capsid; Cell Nucleus; Cell membrane; Cells; Culicidae; Cytolysis; Cytoplasm; Economics; Electron Microscopy; Family member; Fishes; Funding; Genome; Grant; Infection; Invertebrates; Iridoviridae; Iridovirus; Lecithin; Lipids; Morphogenesis; National Center for Research Resources; Nucleotides; Organelles; Phosphatidylinositols; Population; Principal Investigator; Process; Research; Research Infrastructure; Resources; Singapore; Site; Source; Structural Protein; Structure; United States National Institutes of Health; Viral; Virion; Virus; cold blooded vertebrate; cost; interest; macromolecule; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Members of the family Iridoviridae infect a diverse array of invertebrate and cold-blooded vertebrate hosts. A rapidly growing interest in iridoviruses is not only due to economic concerns, but also due to their involvement in amphibian population declines and infection of mosquitoes. Singapore Grouper Iridovirus (SGIV), a major pathogen in fish aquaculture, has a linear dsDNA genome containing 140,131 nucleotide bp. The icosahedral capsid of SGIV has a maximum diameter of 170 nm. There are three major structural proteins, namely ORF072 (50.5kD), ORF075 (20kD) and ORF018 (32kD). The virion also occupies a lipid layer beneath the capsid shell. The lipid layer is prominently rich in phosphatidylcholine and phosphatidylinositol. Virion morphogenesis takes place in morphologically distinct areas of the cytoplasm termed viral assembly sites (AS). When visualized by electron microscopy, AS appear as a clear region within the cytoplasm that is devoid of large cellular organelles and is close to the nucleus. A majority of matured viruses accumulate in paracrystalline arrays within the cytoplasm and are released by cell lysis, while very few are released by budding through cellular plasma membrane. To date, the detail of viral structure and assembly process is not clear.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 家族虹膜科动物科感染了各种各样的无脊椎动物和冷血脊椎动物宿主。对虹膜病毒的兴趣迅速增长，不仅是由于经济问题，而且还因为它们参与了两栖动物人口的下降和蚊子感染。新加坡石斑鱼虹膜病毒（SGIV）是鱼类水产养殖中的主要病原体，其线性DSDNA基因组含有140,131个核苷酸BP。 SGIV的二十面体内衣壳的最大直径为170 nm。 有三种主要的结构蛋白，即ORF072（50.5KD），ORF075（20KD）和ORF018（32KD）。病毒粒子还占据了衣壳壳下方的脂质层。脂质层富含磷脂酰胆碱和磷脂酰肌醇。病毒体形态发生发生在称为病毒组装位点的细胞质的形态不同的区域（AS）。当通过电子显微镜可视化时，如细胞质内的透明区域，没有大细胞细胞器，并且接近细胞核。大多数成熟病毒积聚在细胞质内的二晶阵列中，并通过细胞裂解释放，而很少有人通过细胞质膜萌芽而释放。迄今为止，病毒结构和组装过程的细节尚不清楚。