SINGAPORE GROUPER IRIDOVIRUS (SGIV)

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 虹彩病毒科的成员感染各种无脊椎动物和冷血脊椎动物宿主。人们对虹彩病毒的兴趣迅速增长，这不仅是因为经济上的担忧，也是因为它们参与了两栖动物数量的下降和蚊子的感染。新加坡群斑虹彩病毒(SGIV)是水产养殖业的主要病原，其dsDNA基因组为线性dsDNA，含140,131个核苷酸。SGIV的二十面体衣壳最大直径为170 nm。主要结构蛋白有ORF072(50.5kD)、ORF075(20kD)和ORF018(32kD)。病毒粒子还在衣壳下面占据一层脂层。脂层明显富含磷脂酰胆碱和磷脂酰肌醇。病毒粒子的形态发生在细胞质的不同区域，称为病毒组装部位(AS)。当用电子显微镜观察时，AS在细胞质中出现一个清晰的区域，没有大的细胞器，靠近细胞核。大多数成熟的病毒聚集在细胞质内的晶状排列中，并通过细胞裂解释放，而极少数通过细胞质膜萌发释放。到目前为止，病毒结构和组装过程的细节还不清楚。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Members of the family Iridoviridae infect a diverse array of invertebrate and cold-blooded vertebrate hosts. A rapidly growing interest in iridoviruses is not only due to economic concerns, but also due to their involvement in amphibian population declines and infection of mosquitoes. Singapore Grouper Iridovirus (SGIV), a major pathogen in fish aquaculture, has a linear dsDNA genome containing 140,131 nucleotide bp. The icosahedral capsid of SGIV has a maximum diameter of 170 nm. There are three major structural proteins, namely ORF072 (50.5kD), ORF075 (20kD) and ORF018 (32kD). The virion also occupies a lipid layer beneath the capsid shell. The lipid layer is prominently rich in phosphatidylcholine and phosphatidylinositol. Virion morphogenesis takes place in morphologically distinct areas of the cytoplasm termed viral assembly sites (AS). When visualized by electron microscopy, AS appear as a clear region within the cytoplasm that is devoid of large cellular organelles and is close to the nucleus. A majority of matured viruses accumulate in paracrystalline arrays within the cytoplasm and are released by cell lysis, while very few are released by budding through cellular plasma membrane. To date, the detail of viral structure and assembly process is not clear.