CHLOROPLAST CHAPERONIN

叶绿体伴侣蛋白

• 批准号: 8361102
• 负责人: MANAJIT HAYER-HARTL
• 金额: $ 1.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361102
• 关键词: Architecture; Chaperonin 10; Chaperonin 60; Chloroplasts; Electron Microscopy; Funding; Genome; Grant; Green Algae; Homologous Gene; National Center for Research Resources; Organelles; Plastids; Principal Investigator; Protein Import; Proteins; Research; Research Infrastructure; Resources; Source; Structure; Tandem Repeat Sequences; United States National Institutes of Health; Vascular Plant; chaperonin; cost; macromolecule; stoichiometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The chloroplasts of green algae and higher plants contain a homolog of GroEL called chaperonin (cpn) 60 which functions in the folding of proteins imported into the organelles as well as of proteins encoded by the plastid genome. Cpn60 is distinct from GroEL in that it consists of two divergent but homologous types of subunit, alpha and beta, which share approximately 50% sequence identity. These two subunits occur at roughly 1:1 stoichiometry but their arrangement in the cpn60 tetradecamer and their potential functional significance has remained unclear. Moreover, cpn60 cooperates with two GroES-like co-factors, cpn10 and cpn20. Whereas cpn10 corresponds to GroES in architecture, cpn20 consists of subunits that contain a tandem repeat of cpn10. We propose to use cryo-EM to determine the structure of the chloroplast chaperonin with and without the substrate.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 绿色藻类和高等植物的叶绿体含有GroEL的同系物，称为伴侣蛋白（cpn）60，其在导入细胞器的蛋白质以及质体基因组编码的蛋白质的折叠中起作用。Cpn 60与GroEL的不同之处在于它由两种不同但同源的亚基α和β组成，它们具有大约50%的序列同一性。这两个亚基以大约1：1的化学计量发生，但它们在cpn 60十四聚体中的排列及其潜在的功能意义仍然不清楚。此外，cpn 60还与两个类似GroES的辅因子cpn 10和cpn 20协作。cpn 10在结构上对应于GroES，而cpn 20由含有cpn 10串联重复的亚基组成。我们建议使用cryo-EM来确定叶绿体伴侣蛋白的结构和没有基板。