OVARIAN CANCER PLATELETS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Sood实验室的最新发现表明，血小板可能在卵巢癌生长和转移中起重要作用。 我们假设血小板超微结构的改变反映了血小板功能的改变，从而促进了卵巢癌患者的疾病进展。 因此，为了更好地了解超微结构的变化，可能表示血小板病理学在卵巢癌中，我们建议比较血小板分离的外周血的健康捐助者，从新诊断的卵巢癌患者在超微结构水平使用电子冷冻显微镜。 血小板电子显微镜检查现在临床上用于帮助确定患者是否有与出血风险相关的内在血小板异常。 电子低温显微镜的固有优势使其更适合目前的调查，因为冷冻EM将提供更好的样品保存。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent findings from The Sood laboratory indicate that platelets may play an important role in ovarian cancer growth and metastasis. We hypothesize that platelet ultra-structural changes reflect alterations in platelet function that facilitate disease progression in ovarian cancer patients. Thus, in order to gain better understanding of ultra-structural changes that may denote platelet pathobiology in ovarian cancer, we propose comparing platelets isolated from the peripheral blood of healthy donors to those from newly diagnosed ovarian cancer patients at the ultrastructural level using electron cryomicroscopy. Platelet electron microscopy is now used clinically to help determine if patients have an intrinsic platelet abnormality related to bleeding risk. The inherent advantages of electron cryomicroscopy make it preferable for the current investigation because cryo-EM would afford improved sample preservation.