QUANTITATIVE INTERROGATION OF IN-VIVO MELANOCYTIC LESIONS USING NEAR INFRARED

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的中心目标是开发一种新型的体内光学皮肤成像系统，以定量表征伴随着发育不良转变的皮肤变化。调制成像(MI)仪器使用图案化照明来非侵入性地获取生物组织的亚表面图像。这种非接触式方法能够在大视场范围内快速定量确定组织的光学特性。当与多光谱成像相结合时，多个波长的光学特性提供了组织内的定量测量方法，以确定体内生色团的浓度，包括氧合和脱氧血红蛋白、水、脂肪和黑色素。此外，不同空间频率的图像可以被处理以可视化深度切片的亚表面特征的散射和吸收。我们的假设是，这种多模式MI仪器可以作为一个图像平台，以定量表征良性和恶性黑素细胞皮肤病变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The central aim of this project is to develop a novel in-vivo optical skin imaging system to quantitatively characterize changes in skin that accompany dysplastic transformation. The modulated Imaging (MI) instrument uses patterned illumination to non-invasively obtain subsurface images of biological tissues. This non-contact approach enables rapid quantitative determination of the optical properties of tissues over a wide field-of-view. When combined with multi-spectral imaging, the optical properties at several wavelengths provide quantitative measures within tissues to determine the in-vivo concentrations of chromophores, including oxy- and deoxy-hemoglobin, water, lipid and melanin. Furthermore, images at various spatial frequencies can be processed to visualize depth-sectioned subsurface features in terms of scattering and absorption.Our hypothesis is that this multi-modality MI instrument can serve as an imaging platform for quantitative characterization of benign and malignant melanocytic skin lesions.