THE APPLICATION OF SOLUTION X-RAY SCATTERING TO HIGH-THROUGHPUT STRUCTURAL STUDI

溶液X射线散射在高通量结构研究中的应用

• 批准号: 8362172
• 负责人: EDWARD H SNELL
• 金额: $ 1.04万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362172
• 关键词: Crystallization; Crystallography; Data; Equilibrium; Funding; Goals; Grant; Knowledge; Libraries; Medical Research; Molecular; National Center for Research Resources; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Research Institute; Resources; Roentgen Rays; Sampling; Solutions; Source; Staging; Structure; Techniques; United States National Institutes of Health; beamline; cost; experience; macromolecule; method development; protein complex; structural biology; structural genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We propose to study protein targets emerging out of the purification stage of a high-throughput structural crystallography pipeline by solution x-ray scattering. Our effort will focus on a subset of structural genomics targets that were successfully purified but did not yet provide structural data. Our ultimate goal is to obtain molecular envelopes ab initio for those targets, especially large protein complexes. Additional knowledge obtained on quaternary structure equilibrium and interparticle interaction obtained by x-ray scattering will be highly valuable for refining crystallization conditions. As a control, sample targets that provided structural data will also be studied to establish a baseline. Experience with highthroughput methods development, a sample library of >9000 macromolecules, and raw computing power available at the Hauptman-Woodward Medical Research Institute will be combined with a state-of-the-art beamline 4-2 and 96-well, platebased, small angle X-ray scattering expertise at SSRL to utilize solution x-ray scattering as a complementary, highthroughput technique to obtain valuable structural information.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们建议研究通过溶液X射线散射从高通量结构晶体学管道的纯化阶段出现的蛋白质靶标。我们的努力将集中于成功纯化但尚未提供结构数据的结构基因组学目标的一部分。我们的最终目标是从头算到这些靶标，尤其是大型蛋白质复合物。在通过X射线散射获得的第四纪结构平衡和颗粒间相互作用方面获得的其他知识对于精炼结晶条件非常有价值。作为对照，还将研究提供结构数据的样本目标以建立基线。具有高通量方法开发的经验，> 9000个大分子的样本库以及Hauptman-Woodward医学研究所提供的原始计算能力将与最先进的光束线4-2和96-WELL，基于板块的，基于板块的，小角度的，小角度的X射线X射线散射在SSRL上可利用解决方案X射线构造的散布，以获取X射线散射，以获取供应量的较高的信息，以获取较高的较高的信息。