THE DEVELOPMENT AND APPLICATION OF COMPLEMENTARY STRUCTURAL TECHNIQUES

互补结构技术的开发和应用

• 批准号: 8362304
• 负责人: EDWARD H SNELL
• 金额: $ 0.8万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362304
• 关键词: Affect; Area; Back; Biological; Brain; Cells; Complex; Development; Funding; Grant; Health; Lung; Medical Research; Methods; Modeling; National Center for Research Resources; Nuclear Import; Oxidative Stress; Pathway interactions; Principal Investigator; Proteins; Radiation; Request for Proposals; Research; Research Infrastructure; Research Institute; Resolution; Resources; Source; Structure; Techniques; Transfer RNA; United States National Institutes of Health; Viral; Virus; coping; cost; detector; high throughput technology; irradiation; programs; protein complex; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. At the Hauptman-Woodward Medical Research Institute (HWI) we have growing research areas in structural dynamics, protein complexes, and virus studies. In this proposal we request beamtime for collaborative studies between HWI staff and SSRL staff to develop three complementary areas of biological structural research. Specifically we have used complementary crystallographic and SAXS approaches to resolve dynamical aspects in tRNA recognition complexes. We will use a similar interdisciplinary SAXS and crystallographic approach to determine how different partners complex with the same protein and the affect on biological activity. This project requires synchrotron radiation to extend diffraction resolution and the detector facilities at SSRL to cope with large unit cells. We have a program of research on health-related issues caused by oxidative stress in the brain and lungs. Structural perturbations caused by x-ray irradiation are an important part of these studies. We are developing methods to flag radiation-induced structural changes to extrapolate back to the native structure. We require the use of synchrotron radiation to determine high-resolution detail to develop our radiation model. Finally, we are investigating viral interactions with the nuclear import pathway and developing technologies for high-throughput studies of whole viruses.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在Hauptman-Woodward医学研究所（HWI），我们在结构动力学，蛋白质复合物和病毒研究方面的研究领域不断发展。在此提案中，我们要求Beamtime进行HWI员工和SSRL员工之间的合作研究，以开发三个互补的生物结构研究领域。具体而言，我们已经使用了互补的晶体学和SAXS方法来解决tRNA识别络合物中的动力学方面。我们将使用类似的跨学科SAX和晶体学方法来确定不同的伴侣如何与相同的蛋白质复杂化以及对生物活性的影响。该项目需要同步辐射以扩展衍射分辨率和SSRL的检测器设施以应对大型单元细胞。我们有一项有关由大脑和肺部氧化应激引起的与健康相关问题的研究计划。由X射线照射引起的结构扰动是这些研究的重要组成部分。我们正在开发通过标记辐射引起的结构变化的方法，以推断回到天然结构。我们需要使用同步加速器辐射来确定高分辨率细节以开发我们的辐射模型。最后，我们正在研究与核进口途径的病毒相互作用，并开发了用于整个病毒的高通量研究的技术。