HAUPTMAN-WOODWARD INSTITUTE STRUCTURAL STUDIES OF MEDICALLY RELATED PROJECTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 豪普特曼-伍德沃德医学研究所的一个合作研究小组将几个晶体结构确定项目整合到一个单一的提案中。该提案包括不同阶段的医学相关项目，需要同步加速器时间来延长分辨率，从非常小的晶体中获取数据，并为结构解决方案目的进行数据收集，即需要实验室中可用于异常衍射的波长以外的波长。这项研究包括对非核糖体多肽合成酶的研究，这是抗生素开发的一个潜在领域，以及参与铁载体生产的蛋白质的途径。将收集关于人类屏障自整合因子的超高分辨率数据，以及来自短链氧化还原酶家族的小晶体的数据。还将收集关于两种膜蛋白COX-2和Piox的数据，这两种蛋白都参与了从多不饱和脂肪酸前体生物合成氧基脂。通过合作，最大限度地利用同步加速器的时间和内部专业知识。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A collaborative group of researchers from the Hauptman-Woodward Medical Research Institute have consolidated several crystallographic structure determination projects into a single proposal. The proposal includes medically related projects at various stages requiring synchrotron time to extend resolution, get data from very small crystals and perform data collection for structural solution purposes i.e. requiring wavelengths other than that available in the laboratory for anomalous diffraction. The research includes the study of Non-Ribosomal Peptide Synthetases, a potential area for antibiotic development, and the pathways of proteins involved in siderophore production. Ultra-high resolution data will be collected on human barrier-to-autointegration factor as well as data from small crystals of the short chain oxidoreductase family. Data will also be collected on two membrane proteins, COX-2 and PIOX, both of which are involved in oxylipin biosynthesis from polyunsaturated fatty acid precursors. By collaboration best use is made of the synchrotron time and in-house expertise.