HAUPTMAN-WOODWARD INSTITUTE STRUCTURAL STUDIES OF MEDICALLY RELATED PROJECTS

豪普特曼-伍德沃德研究所医学相关项目的结构研究

• 批准号: 7954279
• 负责人: EDWARD H SNELL
• 金额: $ 0.21万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954279
• 关键词: Anabolism; Antibiotics; Area; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Data; Data Collection; Development; Family; Funding; Grant; Housing; Human; Institutes; Institution; Laboratories; Medical Research; Membrane Proteins; Oxidoreductase; PTGS2 gene; Pathway interactions; Polyunsaturated Fatty Acids; Production; Proteins; Research; Research Institute; Research Personnel; Resolution; Resources; Siderophores; Solutions; Source; Staging; Structure; Synchrotrons; Time; United States National Institutes of Health; barrier-to-autointegration factor; peptide synthase; structural biology; synchrotron radiation; ultra high resolution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A collaborative group of researchers from the Hauptman-Woodward Medical Research Institute have consolidated several crystallographic structure determination projects into a single proposal. The proposal includes medically related projects at various stages requiring synchrotron time to extend resolution, get data from very small crystals and perform data collection for structural solution purposes i.e. requiring wavelengths other than that available in the laboratory for anomalous diffraction. The research includes the study of Non-Ribosomal Peptide Synthetases, a potential area for antibiotic development, and the pathways of proteins involved in siderophore production. Ultra-high resolution data will be collected on human barrier-to-autointegration factor as well as data from small crystals of the short chain oxidoreductase family. Data will also be collected on two membrane proteins, COX-2 and PIOX, both of which are involved in oxylipin biosynthesis from polyunsaturated fatty acid precursors. By collaboration best use is made of the synchrotron time and in-house expertise.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 Hauptman-Woodward医学研究所的一组合作研究人员将几个晶体学结构的确定项目整合到了一个建议中。 该提案包括在各个阶段的医学相关项目，需要同步加速器时间扩展分辨率，从很小的晶体中获取数据并执行数据收集以实现结构解决方案的目的，即需要在实验室中可用于异常衍射的波长。该研究包括研究非核糖体肽合成酶，抗生素发育的潜在区域以及参与铁载体产生的蛋白质的途径。 将收集超高分辨率数据，以人类的屏障到自动化因子以及短链氧化还原酶家族的小晶体的数据收集。 数据还将收集到两个膜蛋白Cox-2和Piox上，它们都参与了来自多不饱和脂肪酸前体的催产素生物合成。通过协作，最佳使用是由同步器时间和内部专业知识制成的。