SOLUTION X-RAY SCATTERING OF DEACTIVATED VIRUS PARTICLES

灭活病毒颗粒的 X 射线散射溶液

• 批准号: 8170368
• 负责人: EDWARD H SNELL
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170368
• 关键词: Arts; Biochemical; Complement; Computer Retrieval of Information on Scientific Projects Database; Crystallization; Data Collection; Encapsulated; Funding; Grant; Institution; Medical Research; Molecular; Process; Research; Research Institute; Research Personnel; Resolution; Resources; Solutions; Source; Synchrotrons; Techniques; United States National Institutes of Health; Virion; Virus; beamline; experience; method development; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. At the Hauptman-Woodward Medical Research Institute (HWI) we have been developing a three part process to enable medium-throughput virus structural studies. This includes; 1) the deactivation of whole virus particles, 2) crystallization and automated encapsulated mounting and, 3) high-throughput synchrotron data collection at ambient conditions. We propose to complement this crystallographic focused approach by conducting SAXS experiments on deactivated virus particles to characterize them, establish low resolution molecular envelopes, and profile them as a function of biochemical conditions (a precursor and useful guide to crystallization). Experience with high-throughput methods development, an industrial collaborator specializing in virus deactivation and purification, and raw computing power available at the Hauptman-Woodward Medical Research Institute will be combined with a state-of-the-art beamline 4-2 and 96-well, platebased, small angle x-ray scattering expertise at SSRL. We will utilize solution x-ray scattering as a complementary, technique to aid us in obtaining valuable structural information.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在Hauptman-Woodward医学研究所（HWI），我们一直在开发一个三部分过程，以实现中等通知病毒结构研究。这包括； 1）整个病毒颗粒的停用，2）结晶和自动封装安装，3）在环境条件下收集高通量同步加速器数据。我们建议通过对停用病毒颗粒进行SAXS实验来补充这种晶体学集中的方法，以表征它们，建立低分辨率的分子信封，并将其介绍为生化条件的函数（前体和有用的结晶指南）。具有高通量方法开发的经验，专门从事病毒失活和纯化的工业合作者以及Hauptman-Woodward医学研究所提供的原始计算能力将与SSRL的SSRL的最先进的4-2和96孔的，板块的小角度X射线散射专业知识相结合。我们将利用解决方案X射线散射作为一种补充技术，以帮助我们获得有价值的结构信息。