SOLUTION X-RAY SCATTERING OF DEACTIVATED VIRUS PARTICLES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 在Hauptman-Woodward医学研究所（HWI），我们一直在开发一个由三部分组成的过程，以实现中等通量的病毒结构研究。这包括：1）全病毒颗粒的灭活，2）结晶和自动封装封固，以及3）在环境条件下的高通量同步加速器数据收集。我们建议通过对灭活病毒颗粒进行SAXS实验来表征它们，建立低分辨率分子包膜，并将它们作为生化条件的函数（结晶的前体和有用的指南）来补充这种晶体学方法。高通量方法开发的经验，专门从事病毒灭活和纯化的工业合作者，以及Hauptman-Woodward医学研究所的原始计算能力将与SSRL最先进的光束线4-2和96孔，板基，小角度X射线散射专业知识相结合。我们将利用溶液X射线散射作为一种补充技术，以帮助我们获得有价值的结构信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. At the Hauptman-Woodward Medical Research Institute (HWI) we have been developing a three part process to enable medium-throughput virus structural studies. This includes; 1) the deactivation of whole virus particles, 2) crystallization and automated encapsulated mounting and, 3) high-throughput synchrotron data collection at ambient conditions. We propose to complement this crystallographic focused approach by conducting SAXS experiments on deactivated virus particles to characterize them, establish low resolution molecular envelopes, and profile them as a function of biochemical conditions (a precursor and useful guide to crystallization). Experience with high-throughput methods development, an industrial collaborator specializing in virus deactivation and purification, and raw computing power available at the Hauptman-Woodward Medical Research Institute will be combined with a state-of-the-art beamline 4-2 and 96-well, platebased, small angle x-ray scattering expertise at SSRL. We will utilize solution x-ray scattering as a complementary, technique to aid us in obtaining valuable structural information.