SOLUTION X-RAY SCATTERING OF DEACTIVATED VIRUS PARTICLES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在豪普特曼-伍德沃德医学研究所(HWI)，我们一直在开发一个三部分的流程，以实现中等吞吐量的病毒结构研究。这包括：1)整个病毒颗粒的失活，2)结晶和自动封装安装，3)在环境条件下的高通量同步加速器数据收集。我们建议通过对灭活的病毒颗粒进行SAXS实验来补充这种结晶学重点方法，以表征它们，建立低分辨率分子膜，并将它们描述为生化条件的函数(结晶的前体和有用的指南)。Hauptman-Woodward医学研究所在高通量方法开发方面的经验、专门从事病毒灭活和纯化的工业合作者以及Hauptman-Woodward医学研究所提供的原始计算能力，将与SSRL最先进的光束线4-2和96孔、平板、小角X射线散射专业知识相结合。我们将利用溶液X射线散射作为补充技术，以帮助我们获得有价值的结构信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. At the Hauptman-Woodward Medical Research Institute (HWI) we have been developing a three part process to enable medium-throughput virus structural studies. This includes; 1) the deactivation of whole virus particles, 2) crystallization and automated encapsulated mounting and, 3) high-throughput synchrotron data collection at ambient conditions. We propose to complement this crystallographic focused approach by conducting SAXS experiments on deactivated virus particles to characterize them, establish low resolution molecular envelopes, and profile them as a function of biochemical conditions (a precursor and useful guide to crystallization). Experience with high-throughput methods development, an industrial collaborator specializing in virus deactivation and purification, and raw computing power available at the Hauptman-Woodward Medical Research Institute will be combined with a state-of-the-art beamline 4-2 and 96-well, platebased, small angle x-ray scattering expertise at SSRL. We will utilize solution x-ray scattering as a complementary, technique to aid us in obtaining valuable structural information.