MECHANISM OF THE ENERGY-STORING REACTIONS IN PHOTOSYNTHETIC ORGANISMS

光合生物的储能反应机制

• 批准号: 8361394
• 负责人: Robert Eugene Blankenship
• 金额: $ 0.52万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361394
• 关键词: Appearance; Bacterial Genome; Binding; Biochemical; Biological; Carrier Proteins; Complex; Electron Spin Resonance Spectroscopy; Electron Transport; Evolution; Fluorescence; Funding; Genomics; Goals; Grant; Horizontal Gene Transfer; Kinetics; Lasers; Life; Mass Spectrum Analysis; Measurement; Membrane; Metabolism; Molecular; Molecular Evolution; Multienzyme Complexes; National Center for Research Resources; Nitrogen Fixation; Organism; Photons; Photosynthesis; Pigments; Principal Investigator; Proteins; Reaction; Recording of previous events; Research; Research Infrastructure; Resources; Source; System; Techniques; Thermodynamics; United States National Institutes of Health; biomedical resource; chemical reaction; cost; flash photolysis; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The chemical reactions leading to long-term energy storage in photosynthetic systems take place within the membrane-bound reaction center complex and an associated group of proteins that make up an electron transport chain. One of the central goals of our research is to identify the molecular parameters responsible for the fact that essentially every photon absorbed by the system leads to stable products. To this end, we do a variety of kinetic, thermodynamic and structural measurements on antenna complexes, reaction centers, electron transport proteins and isolated pigments, using a number of techniques, including ultrafast laser flash photolysis and UV-VIS, fluorescence and electron spin resonance spectroscopies, as well as biochemical and molecular biological analysis. The appearance of photosynthesis and other metabolic processes such as nitrogen fixation had profound effects on the evolution of advanced life on Earth. Our analysis of whole bacterial genomes has revealed that these metabolic processes have complex evolutionary histories, including substantial horizontal gene transfer. We have also used a combination of genomic, molecular evolution techniques and biochemical analysis to identify and characterize previously unknown enzyme complexes with novel activities.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在光合系统中导致长期能量储存的化学反应发生在膜结合反应中心复合物和组成电子传递链的相关蛋白质组内。我们研究的中心目标之一是确定负责这一事实的分子参数，即系统吸收的每个光子基本上都会导致稳定的产物。为此，我们对天线复合物，反应中心，电子传递蛋白和分离的色素进行了各种动力学，热力学和结构测量，使用多种技术，包括超快激光闪光光解和UV-VIS，荧光和电子自旋共振光谱，以及生物化学和分子生物学分析。 光合作用和固氮等其他代谢过程的出现对地球上高级生命的进化产生了深远的影响。我们对整个细菌基因组的分析表明，这些代谢过程具有复杂的进化历史，包括大量的水平基因转移。我们还使用了基因组，分子进化技术和生化分析的组合，以确定和表征以前未知的酶复合物与新的活动。