NATIVE ESI FOR DETERMINING STOICHIOMETRY OF MULTISUBUNIT COMPLEXES

用于确定多亚基复合物化学计量的原生 ESI

• 批准号: 8361430
• 负责人: Robert Eugene Blankenship
• 金额: $ 0.7万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361430
• 关键词: Aerobic; Anaerobic Bacteria; Biological Models; Biological Process; Cells; Chlorobi; Complex; Energy Transfer; Food; Fourier transform ion cyclotron resonance; Funding; Grant; Harvest; Human; LHX2 gene; Light; Mass Spectrum Analysis; National Center for Research Resources; Photosynthesis; Principal Investigator; Process; Proteins; Research; Research Infrastructure; Resources; Source; Theoretical Studies; United States National Institutes of Health; Work; base; biomedical resource; cost; mass spectrometer; photosystem; stoichiometry; thermophilic bacteria

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Photosynthesis is a central biological process that produces all the food and much of the energy used by human beings. An enormous amount of work has been done to understand the structural basis of light harvesting and the efficiency of the energy-transfer process. Several bacterial light-harvesting (LH) complexes, such as LH1, LH2 and Fenna-Matthews-Olson protein (FMO) have served as model systems for structural, spectroscopic and theoretical studies.. The recently discovered, thermophilic bacterium, Candidatus Chloracidobacterium (C.) thermophilum, belongs to the phylum Acidobacteria, and is the only chlorophototroph found in the phylum so far. Surprisingly, the photosystem of the aerobic C. thermophilum closely resembles that of the green sulfur bacteria (GSB), which are strict anaerobes. However, The mechanism that enables the C. thermophilum cells to carry out phototrophy under aerobic conditions is not yet known. FMO proteins of green sulfur bacteria (Chlorobiales) have been extensively studied using a wide range of spectroscopic and theoretical approaches. The new FMO protein is investigated by several approaches including native electrospray in a Bruker 12 tesla FTICR mass spectrometer.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 光合作用是一种中心生物学过程，可产生人类使用的所有食物和许多能量。已经完成了大量的工作，以了解光收集的结构基础和能量转移过程的效率。 Several bacterial light-harvesting (LH) complexes, such as LH1, LH2 and Fenna-Matthews-Olson protein (FMO) have served as model systems for structural, spectroscopic and theoretical studies.. The recently discovered, thermophilic bacterium, Candidatus Chloracidobacterium (C.) thermophilum, belongs to the phylum Acidobacteria, and is the only到目前为止，在门中发现的叶绿素。令人惊讶的是，有氧嗜热杆菌的光系统与严格的厌氧菌相似，类似于绿色硫细菌（GSB）。然而，尚不清楚在有氧条件下使嗜热细胞在有氧条件下进行光疗的机制。绿色硫细菌（叶绿素）的FMO蛋白已通过多种光谱和理论方法进行了广泛的研究。新的FMO蛋白是通过包括Bruker 12 Tesla Fticr质谱仪中的几种方法研究的。