MEASUREMENT OF THE SPECTRAL SHIFT OF A NEW CLASS OF DNA BINDING DYES

新型 DNA 结合染料光谱位移的测量

• 批准号: 8361776
• 负责人: PAUL J SMITH
• 金额: $ 2.24万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361776
• 关键词: Binding; Cell Line; Cells; Collaborations; DNA Binding; Dyes; Flow Cytometry; Fluorescence Spectroscopy; Funding; Grant; Image; In Situ; Life; Measurement; National Center for Research Resources; Performance; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Sorting - Cell Movement; Source; Staining method; Stains; United States National Institutes of Health; Universities; cost; improved; instrument

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In collaboration with Dr. Paul Smith of Cardiff University, we will characterize the emission spectra of a new class of DNA binding dyes that are rapidly cell permeable and excite in the far red. The Cardiff group has characterized these dyes with a combination of conventional flow measurements, fluorescence spectroscopy of bulk samples and live cell spectral imaging. We will use stained cell lines to characterize the performance of the spectral instrument in the NIR region as compared to the other measurements. We will also use the improved PS cytometer to investigate changes in lifetime of these dyes upon DNA binding, potentially enabling separation of bound and unbound flurorophore in situ.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 与卡迪夫大学的保罗·史密斯博士合作，我们将表征一类新型DNA结合染料的发射光谱，这种染料可迅速渗透细胞，并在远红光中激发。卡迪夫小组结合了常规流动测量、大宗样品的荧光光谱和活细胞光谱成像对这些染料进行了表征。我们将使用染色细胞系来表征光谱仪器在近红外区域的性能，并与其他测量结果进行比较。我们还将使用改进的PS细胞仪来研究这些染料在DNA结合时寿命的变化，从而有可能在原位分离结合和未结合的荧光团。