MEASUREMENT OF THE SPECTRAL SHIFT OF A NEW CLASS OF DNA BINDING DYES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 在与卡迪夫大学的Paul Smith博士的合作中，我们将描述一类新的DNA结合染料的发射光谱，这些染料可以快速渗透细胞并在远红外区激发。 卡迪夫研究小组结合常规的流量测量、大量样品的荧光光谱和活细胞光谱成像对这些染料进行了表征。 我们将使用染色的细胞系来表征光谱仪器在NIR区域中与其他测量相比的性能。 我们还将使用改进的PS细胞仪来研究DNA结合后这些染料的寿命变化，从而可能使结合和未结合的荧光团原位分离。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In collaboration with Dr. Paul Smith of Cardiff University, we will characterize the emission spectra of a new class of DNA binding dyes that are rapidly cell permeable and excite in the far red. The Cardiff group has characterized these dyes with a combination of conventional flow measurements, fluorescence spectroscopy of bulk samples and live cell spectral imaging. We will use stained cell lines to characterize the performance of the spectral instrument in the NIR region as compared to the other measurements. We will also use the improved PS cytometer to investigate changes in lifetime of these dyes upon DNA binding, potentially enabling separation of bound and unbound flurorophore in situ.