MEASUREMENT OF THE SPECTRAL SHIFT OF A NEW CLASS OF DNA BINDING DYES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 与卡迪夫大学的保罗·史密斯博士合作，我们将表征一类新型DNA结合染料的发射光谱，这种染料可迅速渗透细胞，并在远红光中激发。卡迪夫小组结合了常规流动测量、大宗样品的荧光光谱和活细胞光谱成像对这些染料进行了表征。我们将使用染色细胞系来表征光谱仪器在近红外区域的性能，并与其他测量结果进行比较。我们还将使用改进的PS细胞仪来研究这些染料在DNA结合时寿命的变化，从而有可能在原位分离结合和未结合的荧光团。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In collaboration with Dr. Paul Smith of Cardiff University, we will characterize the emission spectra of a new class of DNA binding dyes that are rapidly cell permeable and excite in the far red. The Cardiff group has characterized these dyes with a combination of conventional flow measurements, fluorescence spectroscopy of bulk samples and live cell spectral imaging. We will use stained cell lines to characterize the performance of the spectral instrument in the NIR region as compared to the other measurements. We will also use the improved PS cytometer to investigate changes in lifetime of these dyes upon DNA binding, potentially enabling separation of bound and unbound flurorophore in situ.