ZN LOCALIZATION AND SPECIATION IN GRAINS OF ZN-DENSE MUTANT AND LOW PHYTATE MUTA

锌密集突变体和低植酸突变体籽粒中锌的定位和形态

• 批准号: 8362364
• 负责人: Shiqiang Tian
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362364
• 关键词: Accounting; Binding; Biological Availability; Cereals; Child; China; Developing Countries; Diet; Ferritin; Foundations; Funding; Genes; Grant; Health; Human; Ligands; Malnutrition; Micronutrients; Molecular; National Center for Research Resources; Physiology; Plants; Principal Investigator; Radiation; Research; Research Infrastructure; Resources; Rice; Sedum; Soil; Source; Staging; Stress; System; Techniques; United States National Institutes of Health; Work; Zinc; cost; experience; in vivo; micronutrient deficiency; mutant; novel; phytate; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Micronutrient malnutrition is one of major health problems in the world, especially in developing countries. Zinc (Zn) malnutrition in children accounted for 35% in the world, and over 50% in China. Micronutrient deficiencies in human mainly resulted from low concentration and availability of micronutrients in diets. Understanding physiology and molecular physiology of Zn dense in rice grain is of great importance for Zn biofortification in soil-plant system for better human health. In the past more research has been focused on Zn acquisition from soils under deficient stresses, but less is done on the mechanisms of Zn transport and dense into rice grain. It is suggested that Zn transport in plant is regulated by some ZIP transporter genes, and Zn accumulation in grain is associated with some organic ligands like AA and ferritin. However, little information is available on the in vivo Zn localization or complexation in grains, which are the major regulator for Zn bioavailability in rice grain. The objectives of this proposed research are: 1) to determine the dynamic of cellular distribution of Zn in grain of dense rice mutant and low phytate mutant during developing stages using SRXRF technique; 2) to characterize the major binding forms of Zn in grains of both Zn-dense and low-phytate mutants using XAS techniques; Our groups has been working on Zn efficiency in rice and Zn hyperaccumulation in Sedum alfredii for many years, and has a good research foundation, including the novel plant materials (Zn dense grain mutant and low phytate mutant rice), and previous experience of SRXRF and XAS techniques.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 营养不良是当今世界，特别是发展中国家的主要健康问题之一。儿童锌营养不良占世界儿童的35%，我国占50%以上。人类微量营养素缺乏主要是由于膳食中微量营养素的浓度低和可利用性差。了解水稻籽粒锌富集的生理学和分子生理学，对土壤-植物系统锌的生物强化，促进人类健康具有重要意义。以往的研究主要集中在缺锌胁迫下土壤中锌的吸收，而对锌在水稻籽粒中的运输和富集机制的研究较少。研究表明，植物体内锌的转运受某些ZIP转运蛋白基因调控，锌在籽粒中的积累与AA、铁蛋白等有机配体有关。然而，很少有信息可用于在体内锌的定位或络合在粮食，这是主要的调节剂锌的生物利用度在水稻籽粒。本研究的主要目的是：1）利用SRXRF技术研究水稻高密度突变体和低植酸突变体籽粒发育过程中Zn在细胞中的分布动态，2）利用XAS技术研究高密度突变体和低植酸突变体籽粒中Zn的主要结合形式，3）利用X射线荧光光谱技术研究水稻高密度突变体和低植酸突变体籽粒中Zn的主要结合形式。本课题组多年来一直致力于水稻锌效率和东南景天锌超积累的研究，具有良好的研究基础，包括新的植物材料（锌密粒突变体和低植酸突变体水稻）以及SRXRF和XAS技术的先前经验。