BACTERIOPHAGE EPSILON 15
BACTERIOPHAGE EPSILON 15
批准号:
8361071
负责人:
Jonathan Alan King
金额:
$9.81万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-01-01 至 2011-12-31
关键词:
AffectAmberAnimalsBacteriophagesBindingBurkholderiaCD14 AntigenCaliberChromosomesCytoplasmDNAElectron MicroscopyFundingGeneticGenomeGrantHumanImageInfectionLipopolysaccharidesMethodsNational Center for Research ResourcesO AntigensOpen Reading FramesPrincipal InvestigatorReagentResearchResearch InfrastructureResourcesSalmonella entericaSourceStructural ProteinStructureSurfaceUnited States National Institutes of HealthViral ProteinsVirionVirusbacteriophage tailspike proteincostcystic fibrosis patientsimage reconstructionmacromoleculemutantparticlepathogenreceptor
中文摘要
这个子项目是利用资源的许多研究子项目之一。
由NIH/NCRR资助的中心拨款提供。对子项目的主要支持
子项目的首席调查员可能是由其他来源提供的,
包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能
表示该子项目使用的中心基础设施的估计数量,
不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。
噬菌体Epsilon 15是一种感染人/动物的全面性转导噬菌体
病原菌肠炎沙门氏菌血清型。人们对Epsilon 15进行了研究,因为它具有
识别和结合O-抗原及其改变宿主内毒素的能力
溶源状态。负责识别O抗原的病毒蛋白是尾钉蛋白。
该病毒粒子含有至少6种结构蛋白和大约40kb的dsdna。
已知序列的基因组。在序列水平上,最接近的结构蛋白
与伯克霍尔德氏菌的Bcep噬菌体相似,伯克霍尔德氏菌是一种影响囊性纤维化的人类病原体
病人。病毒粒子结构蛋白和染色体一起形成一个具有
质量约为66兆吨,直径约为6500?乔恩·金的实验室
使用质谱学方法鉴定编码病毒粒子的开放阅读框
结构蛋白。这种病毒的结构将揭示出结构的排列
蛋白质,特别是那些更直接参与的亚基的构型
附着在宿主上,并通过DNA进入细胞质。一种遗传方法现在是
正在分离病毒结构蛋白中的琥珀突变体。大分子
在感染这些突变噬菌体期间形成的亚组分将被提纯和成像
通过重建。这些突变的噬菌体也将作为显像剂
病毒与病毒表面脂多糖和受体蛋白的相互作用
主持人。
英文摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Bacteriophage Epsilon15 is a generalized transducing phage infecting the human/animal
pathogen Salmonella enterica serovar Anatum. Epsilon 15 has been studied for its ability to
recognize and bind O-antigen as well as its capacity to alter host lipopolysaccharide in the
lysogenic state. The viral protein responsible for O-antigen recognition is the tailspike protein.
The virion contains at least six structural proteins and an approximately 40 kb dsDNA
genome of known sequence. At the sequence level, the structural proteins most closely
resemble the Bcep phages of Burkholderia, a human pathogen affecting cystic fibrosis
patients. The virion structural proteins and chromosome together make a particle with a
mass of approximately 66 Megadaltons and a diameter of roughly 6500 ¿. Jon King's lab has
used mass spectrometric methods to identify those open reading frames encoding virion
structural proteins. The structure of this virus will reveal the arrangement of structural
proteins and, in particular, the configuration of those subunits more directly involved in
attachment to the host and passage of DNA into the cytoplasm. A genetic approach is now
underway to isolate amber mutants in virus structural proteins. Macromolecular
subassemblies formed during infections with these mutant phage will be purified and imaged
by reconstruction. These mutant phage will also serve as reagents for imaging the
interactions between virus and lipopolysaccharide and receptor proteins at the surface of the
host.
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ALPHA CRYSTALLIN
-
批准号:8361109
-
项目类别:
-
资助金额:$1.23万
-
财政年份:2011
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE SYN 5
-
批准号:8361072
-
项目类别:
-
资助金额:$3.68万
-
财政年份:2011
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE P22
-
批准号:8361056
-
项目类别:
-
资助金额:$4.9万
-
财政年份:2011
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE EPSILON 15
-
批准号:8168543
-
项目类别:
-
资助金额:$8.6万
-
财政年份:2010
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE P22
-
批准号:8168526
-
项目类别:
-
资助金额:$4.3万
-
财政年份:2010
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE SYN 5
-
批准号:8168544
-
项目类别:
-
资助金额:$2.15万
-
财政年份:2010
-
负责人:Jonathan Alan King
-
依托单位:
ALPHA CRYSTALLIN
-
批准号:8168603
-
项目类别:
-
资助金额:$0.43万
-
财政年份:2010
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE P22
-
批准号:7953754
-
项目类别:
-
资助金额:$3.48万
-
财政年份:2008
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE EPSILON 15
-
批准号:7953772
-
项目类别:
-
资助金额:$5.22万
-
财政年份:2008
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE EPSILON 15
-
批准号:7721144
-
项目类别:
-
资助金额:$4.87万
-
财政年份:2007
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE EPSILON 15
-
批准号:7598609
-
项目类别:
-
资助金额:$3.26万
-
财政年份:2006
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE P22
-
批准号:7598581
-
项目类别:
-
资助金额:$1.63万
-
财政年份:2006
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE SYN 5
-
批准号:7598610
-
项目类别:
-
资助金额:$1.63万
-
财政年份:2006
-
负责人:Jonathan Alan King
-
依托单位:
Compounds blocking crystallin aggregation in vitro; path to anti-cataract agents
-
批准号:8078082
-
项目类别:
-
资助金额:$33.64万
-
财政年份:2005
-
负责人:Jonathan Alan King
-
依托单位:
Human gammaD-Crystallin Folding Misfolding & Fibril Form
-
批准号:6925963
-
项目类别:
-
资助金额:$29.97万
-
财政年份:2005
-
负责人:Jonathan Alan King
-
依托单位:
Compounds blocking crystallin aggregation in vitro; path to anti-cataract agents
-
批准号:8663915
-
项目类别:
-
资助金额:$33.35万
-
财政年份:2005
-
负责人:Jonathan Alan King
-
依托单位:
Compounds blocking crystallin aggregation in vitro; path to anti-cataract agents
-
批准号:7885926
-
项目类别:
-
资助金额:$33.77万
-
财政年份:2005
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE EPSILON 15
-
批准号:7357801
-
项目类别:
-
资助金额:$3.01万
-
财政年份:2005
-
负责人:Jonathan Alan King
-
依托单位:
Human gammaD-Crystallin Folding, Misfolding and Fibril Forms
-
批准号:7624621
-
项目类别:
-
资助金额:$29.77万
-
财政年份:2005
-
负责人:Jonathan Alan King
-
依托单位:
BACTERIOPHAGE SYN 5
-
批准号:7357802
-
项目类别:
-
资助金额:$1.51万
-
财政年份:2005
-
负责人:Jonathan Alan King
-
依托单位:
海外基金