BACTERIOPHAGE P22

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 噬菌体P22是沙门氏菌中研究最多的噬菌体之一，在过去的几十年里一直是分子生物学大量遗传学研究的对象，具有显著的转化和转导能力。在病毒成熟为成熟噬菌体的过程中，它以一种称为proapsid的前体开始，内部有支架蛋白，然后dsDNA通过十二个顶点中的一个插入到正在膨胀的衣壳中，支架蛋白退出。尾巴附着在这个顶点上，变得成熟并具有感染力。虽然自2003年以来，P22原衣壳和成熟噬菌体的结构已经被冷冻EM解析到亚纳米(~9埃)分辨率(酱W.et.等，Nat Struct Biol，2003，10：131-5。)，由于低分辨率，大多数结构细节仍然难以捉摸。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacteriophage P22, one of the best studied Salmonella phages, has been the object of tremendous genetic studies for molecular biology in the past several decades and is remarkable for its converting and transducting abilities. During the virus maturation into the mature phage, it starts as a precursor, called procapsid, with scaffolding proteins inside, then the dsDNA inserts into the expanding capsid shell via one of the twelve vertices, and scaffold proteins exit. The tail attaches to this vertex to become mature and infectious. Although both structures of P22 procapsid and mature phage had been solved to subnanometer (~9 Angstrom) resolution by cryo-EM since 2003 (Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.), most of structural details still remain elusive due to the low resolution.