BACTERIOPHAGE P22

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 噬菌体P22是沙门氏菌中研究得最好的噬菌体之一，在过去的几十年里一直是大量分子生物学遗传研究的对象，并且以其转化和转导能力而闻名。在病毒成熟为成熟噬菌体的过程中，它开始作为前体，称为原衣壳，内部有支架蛋白，然后dsDNA通过12个顶点之一插入膨胀的衣壳壳中，支架蛋白退出。尾巴附着在这个顶点上，变得成熟并具有传染性。尽管自2003年以来，P22原衣壳和成熟噬菌体的结构都已通过冷冻-EM解析到亚纳米（~9埃）分辨率（Jiang W. et.例如，Nat Struct Biol，2003，10：131-5.），由于分辨率较低，大多数结构细节仍然难以捉摸。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacteriophage P22, one of the best studied Salmonella phages, has been the object of tremendous genetic studies for molecular biology in the past several decades and is remarkable for its converting and transducting abilities. During the virus maturation into the mature phage, it starts as a precursor, called procapsid, with scaffolding proteins inside, then the dsDNA inserts into the expanding capsid shell via one of the twelve vertices, and scaffold proteins exit. The tail attaches to this vertex to become mature and infectious. Although both structures of P22 procapsid and mature phage had been solved to subnanometer (~9 Angstrom) resolution by cryo-EM since 2003 (Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.), most of structural details still remain elusive due to the low resolution.