BACTERIOPHAGE P22

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 噬菌体P22是沙门氏菌中研究得最好的噬菌体之一， 在过去的几十年里，对分子生物学进行了大量的遗传研究， 以其转换和传导能力而闻名。在病毒成熟为 成熟的噬菌体，它开始作为一个前体，称为原衣壳，与支架蛋白内部，然后 dsDNA通过十二个顶点之一插入扩展的衣壳壳中， 蛋白质退出。尾巴附着在这个顶点上，变得成熟并具有传染性。虽然 P22前衣壳和成熟噬菌体的结构都已被解析到亚纳米级（~9 μ m ）的分辨率（Jiang W. et.例如，Nat Struct Biol，2003，10：131-5.），最 由于分辨率低，结构细节仍然难以捉摸。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bacteriophage P22, one of the best studied Salmonella phages, has been the object of tremendous genetic studies for molecular biology in the past several decades and is remarkable for its converting and transducting abilities. During the virus maturation into the mature phage, it starts as a precursor, called procapsid, with scaffolding proteins inside, then the dsDNA inserts into the expanding capsid shell via one of the twelve vertices, and scaffold proteins exit. The tail attaches to this vertex to become mature and infectious. Although both structures of P22 procapsid and mature phage had been solved to subnanometer (~9 ¿ ) resolution by cryo-EM since 2003 (Jiang W. et. al., Nat Struct Biol, 2003, 10:131-5.), most of structural details still remain elusive due to the low resolution.