STRUCTURE OF A POTASSIUM ION TRANSPORTER

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 离子转运体是一种完整的膜蛋白，它共同运输两种不同的离子物种穿过膜。它们在人体生理中扮演着重要的角色：例如，钠/碘同向转运体负责在甲状腺中积累碘。然而，缺乏对符号如何工作的原子层面的机械理解。这项研究的长期目标是获得大肠杆菌钠/钾同向转运体TrkH的高分辨结构，该结构与调节细胞内蛋白TrkA形成复合体。我们将解决如何实现离子选择性，以及细胞内ATP水平如何调节离子转运功能的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Ion symporters are integral membrane proteins that co-transport two different ion species across the membrane. They play important roles in human physiology: the Sodium/Iodide symporter, for example, is responsible for accumulation of iodide in the thyroid gland. However, an atomic level mechanistic understanding of how symporters work is lacking. The long term goal of this research is to obtain high resolution structures of an E. coli Sodium/Potassium symporter, TrkH, in complex with a regulatory intracellular protein, TrkA. We will address the questions of how the ion selectivity is achieved, and how the function of ion transport is regulated by intracellular ATP level.