STRUCTURE OF A POTASSIUM ION TRANSPORTER

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 离子共转运蛋白是整合的膜蛋白，其跨膜共转运两种不同的离子种类。它们在人体生理学中起着重要作用：例如，钠/碘同向转运体负责甲状腺中碘的积累。然而，缺乏对协同转运体如何工作的原子水平的机械理解。本研究的长期目标是获得高分辨率的E.大肠杆菌钠/钾同向转运体TrkH与细胞内调节蛋白TrkA复合。我们将讨论离子选择性是如何实现的，以及离子转运功能是如何受细胞内ATP水平的调节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ion symporters are integral membrane proteins that co-transport two different ion species across the membrane. They play important roles in human physiology: the Sodium/Iodide symporter, for example, is responsible for accumulation of iodide in the thyroid gland. However, an atomic level mechanistic understanding of how symporters work is lacking. The long term goal of this research is to obtain high resolution structures of an E. coli Sodium/Potassium symporter, TrkH, in complex with a regulatory intracellular protein, TrkA. We will address the questions of how the ion selectivity is achieved, and how the function of ion transport is regulated by intracellular ATP level.