THE PAF1 TRANSCRIPTION ELONGATION COMPLEX

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 转录是围绕聚合酶(通常是RNA聚合酶II)的活性而进行的一项基本的、必不可少的功能，但有效的转录还需要许多额外的调节因子的共同努力。这些因子以各种方式辅助，例如通过减少染色质模板在维持组蛋白上适当的翻译后修饰时提供的物理障碍。五亚基Paf1复合体是一种转录延伸因子，对许多基因的高效转录、活性染色质修饰的维持以及对RNA的正确3‘处理都是必需的。目前还不清楚Paf1是如何协调这些功能的，从序列同源性中几乎没有结构信息或线索来指导我们对Paf1c功能机制的思考。我们希望通过对Paf1复合体以及分离的结构域和具有各种结合伙伴的复合体的结构分析来了解这一点。最终目标是了解Paf1c如何在转录机制的背景下发挥作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcription is a basic, essential function that contered around the activity of a polymerase (often RNA polymerase II), but efficient transcription also requires the concerted efforts of numerous addition regulatory factors. These factors assist in various ways, for example by diminishing the physical barrier provided by the chromatin template as maintaining the appropriate post-translational modifications on the histones. The five subunit Paf1 complex is a transcription elongation factor that is required for the efficient transcription many genes, the maintenance of active chromatin modifications, and for proper 3' processing of RNA. It is not known how Paf1 coordinates these functions and there is little structural information or clues from sequence homology to direct our thinking about the mechanism of Paf1c function. We are hoping to gain insight into this through structural analysis of the Paf1 complex as well as isolated domains and complexes with various binding partners. The end goal is to develop an understanding of how Paf1c functions within the context of the transcription machinery.