THE PAF1 TRANSCRIPTION ELONGATION COMPLEX

PAF1 转录延伸复合物

• 批准号: 8363406
• 负责人: ANDREW Paul VANDEMARK
• 金额: $ 0.19万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363406
• 关键词: Binding; Chromatin; Complex; Crystallography; Elongation Factor; Funding; Genes; Genetic Transcription; Goals; Grant; Histones; Light; Macromolecular Complexes; Maintenance; National Center for Research Resources; Polymerase; Post-Translational Protein Processing; Principal Investigator; RNA Polymerase II; RNA Processing; Research; Research Infrastructure; Resources; Sequence Homology; Source; Synchrotrons; Thinking; Transcription Elongation; United States National Institutes of Health; chromatin modification; cost; insight

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcription is a basic, essential function that contered around the activity of a polymerase (often RNA polymerase II), but efficient transcription also requires the concerted efforts of numerous addition regulatory factors. These factors assist in various ways, for example by diminishing the physical barrier provided by the chromatin template as maintaining the appropriate post-translational modifications on the histones. The five subunit Paf1 complex is a transcription elongation factor that is required for the efficient transcription many genes, the maintenance of active chromatin modifications, and for proper 3' processing of RNA. It is not known how Paf1 coordinates these functions and there is little structural information or clues from sequence homology to direct our thinking about the mechanism of Paf1c function. We are hoping to gain insight into this through structural analysis of the Paf1 complex as well as isolated domains and complexes with various binding partners. The end goal is to develop an understanding of how Paf1c functions within the context of the transcription machinery.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 转录是一个基本的，必不可少的功能，围绕聚合酶（通常是RNA聚合酶II）的活性，但有效的转录也需要许多额外的调节因子的协同努力。 这些因子以各种方式辅助，例如通过减少由染色质模板提供的物理屏障，以维持组蛋白上的适当翻译后修饰。 五亚基Paf 1复合物是一种转录延伸因子，是许多基因有效转录、维持活性染色质修饰和RNA正确3'加工所必需的。 目前尚不清楚Paf 1如何协调这些功能，并且几乎没有结构信息或来自序列同源性的线索来指导我们思考Paf 1c功能的机制。 我们希望通过对Paf 1复合物以及与各种结合伴侣的分离结构域和复合物的结构分析来深入了解这一点。 最终目标是了解Paf 1c如何在转录机制中发挥作用。