THE PAF1 TRANSCRIPTION ELONGATION COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 转录是一个基本的，必不可少的功能，围绕聚合酶（通常是RNA聚合酶II）的活性，但有效的转录也需要许多额外的调节因子的协同努力。 这些因子以各种方式辅助，例如通过减少由染色质模板提供的物理屏障，以维持组蛋白上的适当翻译后修饰。 五亚基Paf 1复合物是一种转录延伸因子，是许多基因有效转录、维持活性染色质修饰和RNA正确3'加工所必需的。 目前尚不清楚Paf 1如何协调这些功能，并且几乎没有结构信息或来自序列同源性的线索来指导我们思考Paf 1c功能的机制。 我们希望通过对Paf 1复合物以及与各种结合伴侣的分离结构域和复合物的结构分析来深入了解这一点。 最终目标是了解Paf 1c如何在转录机制中发挥作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is a basic, essential function that contered around the activity of a polymerase (often RNA polymerase II), but efficient transcription also requires the concerted efforts of numerous addition regulatory factors. These factors assist in various ways, for example by diminishing the physical barrier provided by the chromatin template as maintaining the appropriate post-translational modifications on the histones. The five subunit Paf1 complex is a transcription elongation factor that is required for the efficient transcription many genes, the maintenance of active chromatin modifications, and for proper 3' processing of RNA. It is not known how Paf1 coordinates these functions and there is little structural information or clues from sequence homology to direct our thinking about the mechanism of Paf1c function. We are hoping to gain insight into this through structural analysis of the Paf1 complex as well as isolated domains and complexes with various binding partners. The end goal is to develop an understanding of how Paf1c functions within the context of the transcription machinery.