CRYO-EM RECONSTRUCTION OF THE HUMAN HSP90:HOP COMPLEX

人类 HSP90:HOP 复合体的冷冻电镜重建

• 批准号: 8362473
• 负责人: DAVID A. AGARD
• 金额: $ 0.64万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362473
• 关键词: Biochemical; Cell Cycle; Client; Complex; DNA Sequence Rearrangement; Funding; Grant; Heat-Shock Proteins 90; Human; Humulus; Microscopy; Molecular; Molecular Chaperones; National Center for Research Resources; Nuclear Receptors; Phosphotransferases; Principal Investigator; Proteins; Receptor Cell; Research; Research Infrastructure; Resources; Source; Staging; United States National Institutes of Health; Work; cost; protein complex; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The molecular chaperone Heat Shock Protein 90 (Hsp90) is the central player in a multi- component complex that is required for the folding and activation of numerous essential proteins including nuclear receptors and cell-cycle kinases. Very little is known about 'client' substrate protein and cochaperone interactions on Hsp90 or the structural rearrangements involved in the initial stages of chaperone activity. Previous biochemical work has shown that nuclear receptors and other clients are delivered to an Hsp90:Hop (Hsp90 organizing protein) complex by the Hsp70 chaperone.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 分子伴侣热休克蛋白90（Hsp 90）是一个多功能的热休克蛋白。 这是一个复杂的组成部分，需要折叠和激活许多重要的 蛋白质包括核受体和细胞周期激酶。对“客户”知之甚少 底物蛋白和辅助分子伴侣对热休克蛋白90的相互作用或结构重排 参与分子伴侣活动的初始阶段。先前的生物化学研究表明， 核受体和其他客户端被传递到Hsp 90：Hop（Hsp 90组织蛋白） 由Hsp 70分子伴侣复合。