STRUCTURE OF THE DIMERIZATION DOMAIN OF DIGEORGE CRITICAL REGION 8

DIGEORGE关键区8的二聚化结构域的结构

• 批准号: 8361692
• 负责人: Feng Guo
• 金额: $ 0.34万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361692
• 关键词: Binding; Complex; Development; Dimerization; Disease; Funding; Grant; Heme; Malignant Neoplasms; Mediating; MicroRNAs; Microprocessor; National Center for Research Resources; Principal Investigator; Process; RNA-Binding Proteins; Reporting; Research; Research Infrastructure; Resources; Source; Structure; Transcript; United States National Institutes of Health; cost; nuclease; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Maturation of microRNAs (miRNAs, approximately 22nt) from long primary transcripts [primary miRNAs (pri-miRNAs)] is regulated during development and is altered in diseases such as cancer. The first processing step is a cleavage mediated by the Microprocessor complex containing the Drosha nuclease and the RNA-binding protein DiGeorge critical region 8 (DGCR8). We previously reported that dimeric DGCR8 binds heme and that the heme-bound DGCR8 is more active than the heme-free form. Here, we identified a conserved dimerization domain in DGCR8.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 来自长初级转录物[初级miRNA（pri-miRNA）]的microRNA（miRNAs，约22 nt）的成熟在发育过程中受到调节，并且在疾病如癌症中被改变。第一个处理步骤是由含有Drosha核酸酶和RNA结合蛋白DiGeorge关键区8（DGCR 8）的微处理器复合物介导的切割。我们以前报道，二聚体DGCR 8结合血红素，血红素结合DGCR 8比血红素自由形式更活跃。在此，我们鉴定了DGCR 8中的保守二聚化结构域。