STRUCTURE-FUNCTION STUDIES ON PUF60 & ON NPP4

PUF60 的结构功能研究

• 批准号: 8361666
• 负责人: DEMETRIOS BRADDOCK
• 金额: $ 0.18万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361666
• 关键词: Alternative Splicing; Binding; Complex; Data; Development; Disease; Exons; Family; Funding; Genes; Grant; Human; Malignant Neoplasms; Messenger RNA; National Center for Research Resources; Nucleotide pyrophosphatase; Oligonucleotides; Poly U; Principal Investigator; Process; Protein Isoforms; Proteins; RNA Splicing; Reporting; Research; Research Infrastructure; Resolution; Resources; Source; Structure; United States National Institutes of Health; cost; mRNA Precursor; phosphoric diester hydrolase; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Subproject description #1: PUF60 Poly(U)-binding-splicing factor PUF60 is a human protein involved in alternative splicing, a process by which exons of pre-mRNA are reconnected in multiple ways during RNA-splicing. A single gene, thus a single pre-mRNA, can give rise to different mRNAs, resulting in multiple protein isoforms. We collected diffraction data on PUF60 in complex with various oligonucleotides or proteins. Subproject description #2: NPP4 The nucleotide pyrophosphatase/phosphodiesterase (NPP) family is involved in development & disease, including various cancers, yet no structures of the seven human proteins have been reported to date. We collected high-resolution diffraction data for NPP4 & solved the structure. This is the first human NPP to be structurally determined.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 子项目说明#1： PUF60 多聚（U）结合剪接因子PUF 60是参与选择性剪接的人类蛋白质，选择性剪接是前体mRNA的外显子在RNA剪接期间以多种方式重新连接的过程。 单个基因，即单个前mRNA，可以产生不同的mRNA，从而产生多种蛋白质亚型。 我们收集了与各种寡核苷酸或蛋白质复合的PUF 60的衍射数据。 子项目说明2： NPP4 核苷酸焦磷酸酶/磷酸二酯酶（NPP）家族参与发育和疾病，包括各种癌症，但迄今为止尚未报道这七种人类蛋白质的结构。 我们收集了NPP 4的高分辨率衍射数据并解决了结构。这是第一个在结构上确定的人类NPP。