SPINDLE POLE BODY PHOSPHOPROTEOME IN YEAST MEIOSIS

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 微管组织中心（MTOC）使细胞内的微管成核。 在酵母中，MTOC被称为纺锤体极体（SPB）。 我们已经发现，极光激酶Ipl 1在酵母是必需的SPB复制和分离过程中减数分裂。 同时，polo样激酶Cdc 5在许可SPB复制中起着潜在的作用。 我们计划利用SPB磷酸化蛋白质组技术对减数分裂SPB上的磷酸化残基进行定位。 本研究为深入了解Ipl 1和Cdc 5在减数分裂SPB动态中的蛋白磷酸化机制提供了理论依据。 由于这些调节因子是保守的，并且酵母SPB与中心体在功能上是等同的，并且与中心体共享结构组件，因此这项工作将产生对脊椎动物减数分裂中中心体复制和分离的调节的见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The microtubule-organizing center (MTOC) nucleates microtubules inside the cell. In yeast, the MTOC is called the spindle pole body (SPB). We have found that the Aurora kinase Ipl1 in yeast is required for SPB duplication and separation during meiosis. Meanwhile, the polo-like kinase Cdc5 plays a potential role in licensing SPB duplication. We plan to map phosphoresidues on the meiotic SPB by SPB phosphoproteome. This work could provide insights into the mechanism of protein phosphorylation by Ipl1 and Cdc5 in SPB dynamics during meiosis. Because these regulatory factors are conserved, and the yeast SPB is functionally equivalent to and shares structural components with the centrosome, this work will generate insights into the regulation of centrosome duplication and separation in vertebrate meiosis.