GENOME SEQUENCES AND STRAIN RESOURCES FOR SACCHAROMYCES SENSU STRICTO YEASTS

严格酵母的基因组序列和菌株资源

• 批准号: 8365883
• 负责人: Maitreya J Dunham
• 金额: $ 5.82万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365883
• 关键词: Biology; Code; Collection; Comparative Study; DNA Sequence; Evolution; Funding; Fungal Genome; Genes; Genome; Grant; Haploidy; Investigation; Laboratories; Metabolism; Modeling; National Center for Research Resources; Orthologous Gene; Principal Investigator; Proteins; Publishing; Reading; Research; Research Infrastructure; Resources; Saccharomyces; Saccharomyces cerevisiae; Shotguns; Source; United States National Institutes of Health; Yeasts; cost; gene function; genome sequencing; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. High-quality, well-annotated genome sequences and standardized laboratory strains fuel experimental and evolutionary research. We present improved genome sequences of three species of Saccharomyces sensu stricto yeasts: S. bayanus var. uvarum (CBS 7001), S. kudriavzevii (IFO 1802T and ZP 591), and S. mikatae (IFO 1815T), and describe their comparison to the genomes of S. cerevisiae and S. paradoxus. The new sequences, derived by assembling millions of short DNA sequence reads together with previously published Sanger shotgun reads, have vastly greater long-range continuity and far fewer gaps than the currently available genome sequences. New gene predictions defined a set of 5,261 protein-coding orthologs across the five most commonly studied Saccharomyces yeasts, enabling a re-examination of the tempo and mode of yeast gene evolution and improved inferences of species-specific gains and losses. To facilitate experimental investigations, we generated genetically marked, stable haploid strains for all three of these Saccharomyces species. These nearly complete genome sequences and the collection of genetically marked strains provide a valuable toolset for comparative studies of gene function, metabolism, and evolution, and render Saccharomyces sensu stricto the most experimentally tractable model genus. These resources are freely available and accessible through www.SaccharomycesSensuStricto.org.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 高质量、注释良好的基因组序列和标准化的实验室菌株为实验和进化研究提供了动力。 我们提出了改进的基因组序列的三种严格意义上的酵母菌：S。巴亚努斯湾uvarum（CBS 7001）、S. kudriavzevii（IFO 1802 T和ZP 591）和S.（IFO 1815 T）的基因组，并将其与S.酿酒酵母和酿酒酵母。矛盾的。 通过将数百万个短DNA序列读数与先前发表的桑格鸟枪读数组装在一起而获得的新序列，具有比目前可用的基因组序列大得多的长程连续性和少得多的缺口。 新的基因预测在五种最常研究的酵母菌中定义了一组5，261个蛋白质编码直系同源物，从而能够重新检查酵母基因进化的克里思和模式，并改进对物种特异性增益和损失的推断。 为了便于实验研究，我们产生了遗传标记，稳定的单倍体菌株，所有这三个酵母属物种。 这些几乎完整的基因组序列和基因标记菌株的集合为基因功能、代谢和进化的比较研究提供了有价值的工具集，并使严格意义上的酵母成为实验上最易处理的模型属。 这些资源可通过www.sophomycesSensuiteto.org免费获取。