SUBCOMPLEXES OF THE SPINDLE POLE BODY

主轴极体的子复合体

• 批准号: 8365892
• 负责人: Tracy Davis
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365892
• 关键词: Baculoviruses; Biology; Cells; Centrosome; Collaborations; Complex; Eukaryotic Cell; Funding; Fungal Genome; Grant; Microtubules; National Center for Research Resources; Nuclear; Organelles; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; Work; Yeasts; cost; gamma Tubulin; interest; reconstitution; spindle pole body

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The yeast spindle pole body (SPB) is functionally equivalent to the centrosome in higher eukaryotic cells. The SPB is a multilayered organelle that organizes both the nuclear and cytoplasmic microtubules. We are working toward reconstitution of the microtubule nucleating activity of the SPB. The well characterized small gamma-tubulin complex contains three proteins: Tub4p, Spc97p, and Spc98p. We have expressed all three proteins in baculovirus and found that they readily assemble into a stable, soluble complex. However, the nucleation capacity of the small complex is quite low, suggesting we are missing components required for nucleation. We have recently found that gamma-tubulin and Spc97p are also present as part of larger complex in yeast cells. We are interested in characterizing this complex in collaboration with the Yeast Resource Center.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 酵母纺锤体体（SPB）在功能上等同于较高的真核细胞中的中心体。 SPB是一种多层细胞器，可组织核和细胞质微管。 我们正在努力重建SPB的微管成核活性。 特征良好的小γ-微管蛋白复合物包含三种蛋白质：TUB4P，SPC97P和SPC98P。我们已经在杆状病毒中表达了所有三种蛋白质，发现它们很容易组装成稳定的可溶性复合物。但是，小复合物的成核能力很低，这表明我们缺少成核所需的组件。我们最近发现，γ-微管蛋白和SPC97P也作为酵母细胞中较大复合物的一部分。 我们有兴趣与酵母资源中心合作地描述这一复合体。