SUBCOMPLEXES OF THE SPINDLE POLE BODY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 酵母纺锤体极体(SPB)在功能上等同于高等真核细胞中的中心体。SPB是一个多层细胞器，既有核的也有细胞质的微管。我们正在努力重建SPB的微管成核活性。鉴定良好的小分子微管蛋白复合体包含三种蛋白质：Tub4p、Spc97p和Spc98p。我们已经在杆状病毒中表达了这三种蛋白，并发现它们很容易组装成稳定的、可溶的复合体。然而，小复合体的成核能力相当低，这表明我们缺少成核所需的成分。我们最近发现，在酵母细胞中，γ-微管蛋白和Spc97p也作为更大的复合体的一部分存在。我们有兴趣与酵母资源中心合作描述这一复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The yeast spindle pole body (SPB) is functionally equivalent to the centrosome in higher eukaryotic cells. The SPB is a multilayered organelle that organizes both the nuclear and cytoplasmic microtubules. We are working toward reconstitution of the microtubule nucleating activity of the SPB. The well characterized small gamma-tubulin complex contains three proteins: Tub4p, Spc97p, and Spc98p. We have expressed all three proteins in baculovirus and found that they readily assemble into a stable, soluble complex. However, the nucleation capacity of the small complex is quite low, suggesting we are missing components required for nucleation. We have recently found that gamma-tubulin and Spc97p are also present as part of larger complex in yeast cells. We are interested in characterizing this complex in collaboration with the Yeast Resource Center.