A cryo-electron microscopy training and sample-preparation research proposal

冷冻电子显微镜培训和样品制备研究计划

• 批准号: 8457701
• 负责人: JOHN A MAROHN
• 金额: $ 4.06万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-07 至 2013-08-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8457701
• 关键词: Address; Algorithms; Atomic Force Microscopy; Award; California; Cellular biology; Communities; Computer software; Cryoelectron Microscopy; Development; Electron Microscope; Goals; Grant; Image; Individual; Laboratories; Learning; Left; Macromolecular Complexes; Magnetic Resonance; Magnetic Resonance Imaging; Membrane Proteins; Microfabrication; Microscope; Microscopy; Mining; Molecular; Preparation; Printing; Research; Research Institute; Research Proposals; Research Training; Resolution; Resources; Rest; Sampling; Sampling Studies; Specimen; Structure; Techniques; Technology; Tissues; Training; Universities; Vacuum; Work; career; cold temperature; electron tomography; improved; macromolecule; nanometer; nanoscale; new technology; protein structure; public health relevance; skills; three dimensional structure; tomography; tool

DESCRIPTION (provided by applicant): The PI is developing a "molecular microscope" capable of taking nanometer-resolution magnetic resonance images of individual macromolecules. In support of this work, the PI will be trained in state-of-the- art cryo-electron microscopy, and will carry out research to develop improved approaches for preparing fragile biomacromolecules for study at low temperatures in vacuum. The proposed research in sample preparation will make it much easier to study a wider range of samples by both cryo-electron microscopy and nanometer-resolution magnetic resonance imaging.

描述（由申请人提供）：PI正在开发一种能够拍摄单个大分子的纳米分辨率磁共振图像的“分子显微镜”。为了支持这项工作，PI将接受最先进的冷冻电子显微镜的培训，并将开展研究，以开发改进的方法，用于制备脆弱的生物大分子，以便在真空低温下进行研究。拟议中的样品制备研究将使通过低温电子显微镜和纳米分辨率磁共振成像研究更广泛的样品变得更加容易。