Cantilever Magnetic Resonance of Biomolecules

生物分子的悬臂磁共振

• 批准号: 8298544
• 负责人: JOHN A MAROHN
• 金额: $ 32.5万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8298544
• 关键词: Address; Atomic Force Microscopy; Binding; Biological; Biological Process; Biology; Biomedical Technology; Cell membrane; Cell physiology; Chemicals; Detection; Device or Instrument Development; Disease; Electron Spin Resonance Spectroscopy; Electrons; Friction; Goals; Image; In Vitro; Label; Learning; Lipid Bilayers; Location; Macromolecular Complexes; Magnetic Resonance; Magnetic Resonance Imaging; Magnetism; Maps; Medicine; Microscope; Molecular; NIH Program Announcements; Nanotechnology; National Institute of Biomedical Imaging and Bioengineering; National Institute of General Medical Sciences; Noise; Nuclear Magnetic Resonance; Preparation; Property; Proteins; Protons; Research Personnel; Resolution; Sampling; Scanning; Scheme; Speed; Spin Labels; Surface; Technology; Temperature; Time; Vacuum; Work; cantilever; cryogenics; density; design; electron beam lithography; experience; graduate student; improved; induced pluripotent stem cell; instrument; macromolecule; nanofabrication; nanoscale; nanoscience; nanostructured; novel strategies; protein complex; research study; response; single molecule; three dimensional structure; tool

New tools for determining the three dimensional structure of single macromolecules and macromolecular complexes are urgently needed. The goal of this R01 renewal proposal is to create a "molecular microscope" capable of imaging the proton density of, or mapping the locations of electron spin labels in, a single copy of a protein or macromolecular complex. We will develop a molecular microscope capable of scanned-probe nuclear magnetic resonance (NMR) imaging and electron spin resonance (ESR) imaging. Instrument development will be directed towards the study of native and spin-labeled macromolecular complexes bound to a lipid bilayer or located in a cell membrane. Our specific aims are: (1) To exploit magnetic-tipped cantilevers and force-gradient detection of magnetic resonance to observe electron spin resonance (ESR) from a single copy of a nitroxide-labeled protein and to observe nuclear magnetic resonance (NMR) from a few hundred protons in a biomolecule; (2) To understand and mitigate (via sample preparation, cantilever design, and spin modulation schemes) excess force and force- gradient noise experienced by ultrasensitive cantilevers near surfaces in vacuum at cryogenic temperatures and to learn to prolong spin coherence time by giving more perfect spin flips in magnetic resonance force microscopy; and (3) To develop time- domain Fourier image encoding to increase the imaging speed of scanned-probe magnetic resonance.

测定单个大分子和大分子三维结构的新工具 迫切需要大分子络合物。本R01更新提案的目标是 是创造一种“分子显微镜”，能够成像质子密度，或 绘制电子自旋标记的位置图、蛋白质的单个副本或 大分子复合体。 我们将开发一种能够扫描探针核磁的分子显微镜 核磁共振成像和电子自旋共振成像。仪表 发展方向将是研究原生的和自旋标记的 结合在脂质双层上或位于细胞膜上的大分子复合体。 我们的具体目标是：(1)开发磁端悬臂梁和力梯度 磁共振检测，以观察电子自旋共振(ESR)从单个 复制一种氮氧化物标记的蛋白质并观察核磁共振 从生物分子中的几百个质子；(2)理解和减轻(通过样品 准备、悬臂设计和自旋调制方案)多余的力和力- 真空条件下近表面超灵敏悬臂梁的梯度噪声 低温温度，并学会通过给出更多 核磁共振力显微镜中完美的自旋翻转；以及(3)开发时间- 提高扫描探头磁成像速度的域傅里叶图像编码 共鸣。

项目成果

Magnetic Resonance Force Microscopy Detected Long-Lived Spin Magnetization.

• DOI: 10.1109/tmag.2013.2239268
• 发表时间: 2013-07
• 期刊: IEEE transactions on magnetics
• 影响因子: 2.1
• 作者: Chen L;Longenecker JG;Moore EW;Marohn JA
• 通讯作者: Marohn JA

Dielectric fluctuations in force microscopy: noncontact friction and frequency jitter.

• DOI: 10.1063/1.2932254
• 发表时间: 2008-06
• 期刊: The Journal of chemical physics
• 作者: Showkat M. Yazdanian;J. Marohn;Roger F Loring

Quantifying electric field gradient fluctuations over polymers using ultrasensitive cantilevers.

使用超灵敏悬臂梁量化聚合物上的电场梯度波动。

• DOI: 10.1021/nl9004332
• 发表时间: 2009
• 期刊: Nano letters
• 影响因子: 10.8
• 作者: Yazdanian,ShowkatM;Hoepker,Nikolas;Kuehn,Seppe;Loring,RogerF;Marohn,JohnA
• 通讯作者: Marohn,JohnA

Evading surface and detector frequency noise in harmonic oscillator measurements of force gradients.

在力梯度的谐振子测量中避免表面和探测器频率噪声。

• DOI: 10.1063/1.3465906
• 发表时间: 2010
• 期刊: Applied physics letters
• 影响因子: 4
• 作者: Moore,EricW;Lee,Sanggap;Hickman,StevenA;Harrell,LeeE;Marohn,JohnA
• 通讯作者: Marohn,JohnA

High-gradient nanomagnets on cantilevers for sensitive detection of nuclear magnetic resonance.

• DOI: 10.1021/nn3030628
• 发表时间: 2012-11-27
• 期刊: ACS NANO
• 影响因子: 17.1
• 作者: Longenecker, Jonilyn G.;Mamin, H. J.;Senko, Alexander W.;Chen, Lei;Rettner, Charles T.;Rugar, Daniel;Marohn, John A.
• 通讯作者: Marohn, John A.