Plug-and-Play, Sensor-based, NanoLC/ESI Technology for Proteomics and Glycomics

用于蛋白质组学和糖组学的即插即用、基于传感器的 NanoLC/ESI 技术

• 批准号: 8426133
• 负责人: Colleen K Van Pelt
• 金额: $ 40万
• 依托单位: CORSOLUTIONS, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8426133
• 关键词: Address; Area; Back; Biological; Biological Sciences; Chemicals; Collaborations; Communities; Complex; Complex Mixtures; Computer software; Coupled; Custom; Data; Databases; Development; Device Designs; Devices; Diagnosis; Diagnostic; Dimensions; Docking; Electronics; Enhancement Technology; Gases; Generations; Genomics; Goals; Heating; Intervention; Laboratories; Laboratory Study; Length; Letters; Licensing; Liquid Chromatography; Marketing; Mass Spectrum Analysis; Monitor; Performance; Phase; Play; Plumbing; Polysaccharides; Post-Translational Protein Processing; Process; Proteins; Proteomics; Pump; Reading; Reproducibility; Resolution; Sample Size; Sampling; Scientist; Site; Small Business Innovation Research Grant; Solutions; Standardization; System; Techniques; Technology; Variant; Weight; Work; base; commercialization; complex biological systems; falls; improved; liquid chromatography mass spectrometry; mass spectrometer; nano; nano-electrospray; nanofluidic; novel; pressure; prevent; prototype; public health relevance; repaired; seal; sensor; tool; user-friendly; wound

DESCRIPTION (provided by applicant): Nano-liquid chromatography is a desirable technique because when coupled with mass spectrometry it can identify many of the components of a very complex proteomic or glycomic sample including subtle chemical variations within the sample, and can also obtain quantitative information. The technique is renowned for its high chromatographic resolution, high sensitivity, and small sample size requirement. But the technique remains cumbersome as it suffers from several limitations including system reproducibility issues created by user inabilities in column and/or spray emitter set-up, difficulty making nanofluidic connections, and difficulty troubleshooting system malfunctions. Others have tried to simplify this underutilized technique through primarily chip-based approaches, but have unfortunately compromised the technique's resolution and sensitivity. However, in a Phase I project, CorSolutions developed a robust, reliable, user-friendly nanoLC/ESI device that addresses these limitations of conventional nanoLC/ESI systems while still maintaining state-of- the-art performance, and is able to operate at elevated UHPLC pressures. These Phase I prototypes are the first plug-and-play nanoLC/ESI devices which do not compromise the high chromatographic resolution and sensitivity for which nanoLC is renowned. But although the technology developed in Phase I offers great improvements over conventional approaches, further developments are required for the technology to reach its large commercialization potential. The goals of the Phase II are to build upon the compression connection, plug-and-play technology developed in Phase I, in order to ready the technology for commercialization. This includes development of a cartridge-based insert for the column, allowing a column of any length to be wound in a small footprint. This enhancement will allow the overall device dimension to be substantially reduced. Additionally an emitter changer will be added, permitting the device to quickly exchange an emitter, in less than 1 sec, without need for user intervention. A spray sensor and leak sensors will be incorporated into the device with "smart" software to continuously monitor the sensor read back. The software will be capable of diagnosing system malfunctions and attempting automated repair such as an emitter change, greatly simplifying the troubleshooting process. Furthermore the ability to deliver nebulization gas will be incorporated into the device. These enhancements will allow for product commercialization. We see this simple, intuitive product becoming an integral part of biological laboratories, allowing standardization of proteomic and glycomic analyses. And most importantly the product will allow biologists to benefit from the highly informative nanoLC/MS data, enabling scientific breakthroughs and greater understanding of complex biological systems. This significant reduction in complexity of nanoLC will also allow for the technique to infiltrate deeper into the life sciences.

描述（由申请人提供）：纳米液相色谱法是一种理想的技术，因为当与质谱法结合时，它可以鉴定非常复杂的蛋白质组或糖组学样品的许多组分，包括样品内的细微化学变化，并且还可以获得定量信息。该技术以其高色谱分辨率、高灵敏度和小样品量要求而闻名。但是该技术仍然很麻烦，因为它受到几个限制，包括由用户在柱和/或喷雾发射器设置中的不能力产生的系统再现性问题，难以进行纳米流体连接，以及难以排除系统故障。其他人试图通过主要基于芯片的方法来简化这种未充分利用的技术，但不幸的是，这些方法损害了该技术的分辨率和灵敏度。然而，在第一阶段项目中，CorSolutions开发了一种坚固、可靠、用户友好的nanoLC/ESI设备，解决了传统nanoLC/ESI系统的这些局限性，同时仍然保持了最先进的性能，并且能够在升高的UHPLC压力下运行。这些第一阶段的原型是第一个即插即用的nanoLC/ESI设备，不损害高色谱分辨率和灵敏度，其中nanoLC是著名的。但是，尽管第一阶段开发的技术比传统方法有了很大的改进，但该技术还需要进一步开发才能实现其巨大的商业化潜力。 第二阶段的目标是建立在第一阶段开发的压缩连接、即插即用技术的基础上，以便为该技术的商业化做好准备。这包括开发用于柱的基于嵌件的插入件，允许任何长度的柱以小的占地面积缠绕。这种增强将允许整体器件尺寸显著减小。此外，还将添加发射器更换器，允许设备在不到1秒的时间内快速更换发射器，而无需用户干预。喷雾传感器和泄漏传感器将与“智能”软件集成到设备中，以持续监控传感器的回读。该软件将能够诊断系统故障，并尝试自动修复，如发射器的变化，大大简化了故障排除过程。此外，输送雾化气体的能力将被纳入器械。这些改进将允许产品商业化。 我们看到这个简单、直观的产品成为生物实验室不可或缺的一部分，使蛋白质组学和糖组学分析标准化。最重要的是，该产品将使生物学家能够从信息丰富的nanoLC/MS数据中受益，从而实现科学突破并更好地了解复杂的生物系统。nanoLC复杂性的显著降低也将使该技术更深入地渗透到生命科学中。