Glycomics Laboratory for the Early Detection of Epithelial Ovarian Cancer

早期检测上皮性卵巢癌的糖组学实验室

• 批准号: 8351959
• 负责人: KAREN L. ABBOTT
• 金额: $ 35.6万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-03 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8351959
• 关键词: Antibodies; Antibody Formation; Biological Assay; Biological Markers; Breast Carcinoma; COOH-terminal signal transamidase; Cancer Patient; Carbohydrates; Cell Adhesion; Cell Survival; Cells; Clinic; Clinical; Complex; Data; Detection; Development; Diagnosis; Diagnostic; Disease; Early Diagnosis; Epithelial ovarian cancer; Glycoconjugates; Glycoproteins; Goals; Grant; Greater sac of peritoneum; Growth; Gynecologic; Immune; Immunohistochemistry; Immunology; Knowledge; Laboratories; Lead; Letters; Link; Malignant - descriptor; Malignant Epithelial Cell; Malignant Neoplasms; Malignant neoplasm of ovary; Mammalian Oviducts; Marker Discovery; Mass Spectrum Analysis; Measures; Membrane; Membrane Proteins; Microscopic; Modification; Molecular; Ovary; Pennsylvania; Phase; Plasma; Polysaccharides; Post-Translational Protein Processing; Proteins; Proteomics; Protocols documentation; Recurrence; Research; Research Personnel; Role; Sampling; Screening for Ovarian Cancer; Screening procedure; Sensitivity and Specificity; Serum; Site; Specialized Center; Specificity; Stage at Diagnosis; Staging; Survival Rate; Symptoms; Technology; Thrombospondin 1; Time; Tissues; Tumor Cell Invasion; Tumor Expansion; Tumor Tissue; United States; Universities; Validation; Woman; advanced disease; anticancer research; assay development; base; cancer cell; catalyst; glycoprotein structure; glycosylation; human tissue; improved; insight; killings; migration; multidisciplinary; neoplastic cell; novel; periostin; pre-clinical; response; sugar; tumor

DESCRIPTION (provided by applicant): Ovarian cancer is a silent killer with few early symptoms and advanced disease present at the time of diagnosis. This cancer is the most lethal of all gynecologic malignancies with over 20,000 new cases diagnosed each year. The 5 year survival rates for ovarian cancer dramatically improve when the disease is diagnosed at an early stage. Therefore, the long term goal of the Glycomics Laboratory for the Early Detection of Epithelial Ovarian Cancer is to apply the most advanced technologies toward the goal of identifying glycoprotein and glycan markers that can detect ovarian cancer early. We are a multidisciplinary team with expertise in proteomics, glycomics, and immunology from investigators at the Penn Ovarian Cancer Research Center at the University of Pennsylvania (UPENN) and the Complex Carbohydrate Research Center (CCRC) at the University of Georgia. The co-investigators at the CCRC specialize in the mass spectrometry identification of glycan and glycoprotein biomarkers from complex human tissue and serum samples. The co- investigators at UPENN specialize in preclinical validations, antibody production, and the development of biomarkers for ovarian cancer. The aims of this application are (1) Assay development with phase II and phase III validations of previously identified glycosylation modifications on identified biomarkers for EOC, (2) Glycomic identification of new EOC glycoprotein biomarkers, (3) Functional studies to understand the role of GPI anchored protein modification in the development and progression of EOC. PUBLIC HEALTH RELEVANCE: The research proposed is focused on applying the most advanced technologies for the identification of proteins that carry unique sugar (glycan) molecules specific for ovarian cancer. The detection of the glycan present on these proteins will provide a sensitive, specific assay for the early detection of epithelial ovarian cancer. Early detection of ovarian cancer will save lives.

描述（由申请人提供）：卵巢癌是一种沉默的杀手，早期症状很少，诊断时存在晚期疾病。这种癌症是所有妇科恶性肿瘤中最致命的，每年诊断出超过20，000例新病例。卵巢癌的5年生存率在疾病早期诊断时显着提高。因此，糖组学实验室早期检测上皮性卵巢癌的长期目标是应用最先进的技术，以确定可以早期检测卵巢癌的糖蛋白和聚糖标志物。我们是一个多学科团队，拥有蛋白质组学，糖组学和免疫学方面的专业知识，来自宾夕法尼亚大学（UPENN）宾夕法尼亚卵巢癌研究中心和格鲁吉亚大学复杂碳水化合物研究中心（CCRC）的研究人员。CCRC的合作研究者专门从事从复杂的人体组织和血清样本中鉴定聚糖和糖蛋白生物标志物的质谱法。UPENN的联合研究人员专门从事临床前验证、抗体生产和卵巢癌生物标志物的开发。本申请的目的是：（1）对先前鉴定的EOC生物标志物上的糖基化修饰进行II期和III期验证的试验开发，（2）新EOC糖蛋白生物标志物的糖组学鉴定，（3）了解GPI锚定蛋白修饰在EOC发展和进展中的作用的功能研究。 公共卫生相关性：这项研究的重点是应用最先进的技术来鉴定携带卵巢癌特异性糖（聚糖）分子的蛋白质。检测这些蛋白质上存在的聚糖将为上皮性卵巢癌的早期检测提供灵敏、特异的检测方法。早期发现卵巢癌可以挽救生命。