Glycomics Laboratory for the Early Detection of Epithelial Ovarian Cancer

描述（申请人提供）：卵巢癌是一种沉默的杀手，在诊断时很少出现早期症状和晚期疾病。这种癌症是所有妇科恶性肿瘤中最致命的，每年有超过20,000例新诊断病例。如果卵巢癌在早期阶段得到诊断，其5年生存率会显著提高。因此，早期检测上皮性卵巢癌的糖组学实验室的长期目标是应用最先进的技术来鉴定可以早期检测卵巢癌的糖蛋白和聚糖标记物。我们是一个多学科团队，在蛋白质组学，糖组学和免疫学方面具有专业知识，来自宾夕法尼亚大学宾夕法尼亚卵巢癌研究中心（UPENN）和乔治亚大学复杂碳水化合物研究中心（CCRC）的研究人员。CCRC的合作研究人员专门从事复杂人体组织和血清样本中多糖和糖蛋白生物标志物的质谱鉴定。UPENN的合作研究人员专门从事临床前验证，抗体生产和卵巢癌生物标志物的开发。该申请的目的是：(1)开展II期和III期试验，验证先前鉴定的EOC生物标志物上的糖基化修饰，(2)鉴定新的EOC糖蛋白生物标志物，(3)进行功能研究，以了解GPI锚定的蛋白质修饰在EOC的发展和进展中的作用。

DESCRIPTION (provided by applicant): Ovarian cancer is a silent killer with few early symptoms and advanced disease present at the time of diagnosis. This cancer is the most lethal of all gynecologic malignancies with over 20,000 new cases diagnosed each year. The 5 year survival rates for ovarian cancer dramatically improve when the disease is diagnosed at an early stage. Therefore, the long term goal of the Glycomics Laboratory for the Early Detection of Epithelial Ovarian Cancer is to apply the most advanced technologies toward the goal of identifying glycoprotein and glycan markers that can detect ovarian cancer early. We are a multidisciplinary team with expertise in proteomics, glycomics, and immunology from investigators at the Penn Ovarian Cancer Research Center at the University of Pennsylvania (UPENN) and the Complex Carbohydrate Research Center (CCRC) at the University of Georgia. The co-investigators at the CCRC specialize in the mass spectrometry identification of glycan and glycoprotein biomarkers from complex human tissue and serum samples. The co- investigators at UPENN specialize in preclinical validations, antibody production, and the development of biomarkers for ovarian cancer. The aims of this application are (1) Assay development with phase II and phase III validations of previously identified glycosylation modifications on identified biomarkers for EOC, (2) Glycomic identification of new EOC glycoprotein biomarkers, (3) Functional studies to understand the role of GPI anchored protein modification in the development and progression of EOC.