Molecular signatures associated with prostatic inflammation in rodent models.

匹兹堡大学跨学科规划中心提出的这份竞争性续签申请 良性泌尿学研究(IR-BU)是为了研究与前列腺癌相关的分子标记 由于炎症在良性前列腺增生症中的重要性，一组研究人员 拥有不同的专业知识，包括内分泌学、病理学、泌尿学和药理学，将共同努力 确定炎症诱导的分子和细胞变化的异同和/或 在这个多学科的项目中，在啮齿动物和人类之间的前列腺外。研究假设 前列腺炎症可以诱导与人类BPH相关的分子和细胞变化。这 这一假说得到了当前资助期间的发现的支持，即福尔马林导致的低级别 炎症增强福尔马林注射大鼠前列腺模型雄激素反应基因的表达 我们之前的研究发现，雄激素反应基因在人BPH上皮细胞中的表达升高 细胞。以福尔马林诱导的大鼠前列腺炎为模型，我们建议确定黄连胶囊的作用。 前列腺炎对膀胱和传入功能的影响(特异靶1)，炎性细胞因子谱 前列腺和尿液(特定目标2)和前列腺上皮-间充质转化(特定目标3)。这个 该研究项目的成功将在啮齿类动物中确定相关的靶点和信号通路 炎症导致的前列腺组织动态平衡改变是人类良性前列腺增生症的特征。 IR-BU建立了一个行政核心，其中包括一个执行委员会和一个内部 咨询委员会，将通过项目审查、促进 合作和教育丰富。核心还将负责分配和监督 IR-BU资源。通过支持教育充实和研究项目，行政部门 CORE将帮助IR-BU作为一种资源融入大学社区，并帮助 吸引新的研究人员进入良性前列腺增生症领域。

This competing renewal application from the University of Pittsburgh Planning Center for Interdisciplinary Research in Benign Urology (IR-BU) is proposed to study "Molecular signatures associated with prostatic inflammation in rodent models". Because of the importance of inflammation in BPH, a team of investigators with different expertise including endocrinology, pathology, urology, and pharmacology will work together to determine similarities and dissimilarities in inflammation-induced molecular and cellular changes in and/or outside the prostate between rodents and humans in this multidisciplinary project. The research hypothesis is that prostatic inflammation can induce molecular and cellular changes associated with human BPH. This hypothesis is supported by the finding during the current funding period that formalin-induced low grade inflammation enhanced expression of androgen-responsive genes in the formalin-injection rat prostate model and our previous finding that androgen-responsive gene expression is elevated in human BPH epithelial cells. Using formalin-induced rat prostate inflammation as a model, we propose to determine the effect of prostatic inflammation on bladder and afferent function (Specific Aim 1), inflammatory cytokine profile in the prostate and urine (Specific Aim 2), and prostatic epithelial-mesenchymal transition (Specific Aim 3). The success of this research project will identify relevant targets and signaling pathways in rodents that respond to inflammation to generate altered prostate tissue homeostasis characteristic of BPH in humans. The IR-BU has established an Administrative Core, which includes an Executive Committee and an Internal Advisory Committee, will provide strong administrative support through project review, promoting collaborations, and educational enrichment. The Core will also be responsible for allocation and oversight of IR-BU resources. Through supporting the educational enrichment and research project, the Administrative Core will help to both integrate the IR-BU into the University community by serving as a resource and to attract new investigators to the field of BPH.