权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Stomate-based innate immunity against bacterial infection in Arabidopsis

拟南芥基于气孔的针对细菌感染的先天免疫

基本信息

批准号：
8237064
负责人：
SHENG YANG HE
金额：
$ 35.85万
依托单位：
MICHIGAN STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-01 至 2015-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8237064
关键词：
Abscisic Acid Agriculture Animals Arabidopsis Area Bacteria Bacterial Infections Bacterial Toxins Basic Science Behavior Biochemical Biological Assay Carbon Dioxide Cells Complex Development Effectiveness Escherichia coli O157 Flagellin Food Contamination Food Poisoning Future Genetic Goals Health Homeostasis Hormones Host Defense Human Humidity Immune response Infection Knowledge Light Lipopolysaccharides Mediating Microbe Microscopy Molecular Molecular Genetics Natural Immunity Nitric Oxide Output Pathogenesis Pattern Peptides Perception Phosphotransferases Plant Epidermis Plant Growth Regulators Plant Leaves Plant Model Plants Production Prophylactic treatment Pseudomonas syringae Public Health Research Role Salicylic Acids Signal Transduction Signal Transduction Pathway Source Stomas Stress Surface Testing Time Tomatoes Vegetables Virulence Virulence Factors Virulent Water base cell type coronatine improved movie novel pathogen pathogenic bacteria plant poison prevent public health relevance receptor response ubiquitin-protein ligase uptake

项目摘要

DESCRIPTION (provided by applicant): Innate immunity in animals and plants can be activated by highly conserved pathogen/microbe- associated molecular patterns (PAMPs/MAMPs), such as bacterial flagellin and lipopolysaccharides (LPSs). Recent studies provide evidence that plant stomata, pores in the epidermis of plants, function in innate immunity against the entry of bacteria into the plant. Specifically, stomata close in response to Escherichia coli O157:H7 and Pseudomonas syringae pv. tomato (Pst) DC3000 or bacterial PAMPs. Perception of PAMPs by guard cells, the pair of cells that forms the stomatal pore, requires a flagellin receptor as well as production and signaling of the plant hormones abscisic acid and salicylic acid. The virulent plant pathogen Pst DC3000 produces the phytotoxin coronatine (COR) to suppress stomatal closure as a virulence strategy. Collectively, these results suggest an important role of stomata in modulating the interactions of plants with plant and human pathogenic bacteria in the phyllosphere (the leaf surface). The long-term goal of this project is to elucidate the signal transduction pathway leading to stomate- based defense and to dissect the mechanism by which the bacterial toxin COR inhibits this novel host defense. The central hypotheses to be tested are that (i) stomate-based defense is an integral part of the plant's natural immune response to restrict the entry of bacteria, including human pathogenic bacteria, and (ii) to be successful, plant pathogens must rely on specific virulence factors or environmental conditions to suppress stomate-based defense. An integrative approach involving molecular genetics, microscopy, and biochemical and pathogenesis assays will be used. The proposed research will contribute to the fundamental knowledge of innate immunity and bacterial pathogenesis in plants and will increase our understanding of the molecular basis of plant-human pathogen interactions in the phyllosphere. Human pathogen contamination of fresh vegetables is a major public health concern and a potential bio-terrorist threat as a source of food poisoning. PUBLIC HEALTH RELEVANCE: This proposal describes basic research on the molecular basis of innate immunity of the model plant Arabidopsis to plant and human pathogenic bacteria. Food contamination with human pathogens is a major concern to public health. This research has the potential to provide a mechanistic understanding of plant defense and human pathogen contamination of fresh produce and to guide future development of appropriate prevention measures.

描述（由申请人提供）：动物和植物中的先天免疫可被高度保守的病原体/微生物相关分子模式（PAMP/MAMP）激活，如细菌鞭毛蛋白和脂多糖（LPS）。最近的研究提供证据表明，植物气孔，植物表皮上的孔，在先天免疫中起作用，以防止细菌进入植物。具体而言，气孔关闭响应大肠杆菌O 157：H7和假单胞菌pv.番茄（Pst）DC 3000或细菌PAMP。保卫细胞（形成气孔的一对细胞）对PAMPs的感知需要鞭毛蛋白受体以及植物激素脱落酸和水杨酸的产生和信号传导。有毒植物病原菌Pst DC 3000产生植物毒素冠菌素（COR）以抑制气孔关闭作为毒力策略。总的来说，这些结果表明，气孔在调节植物与植物和人类病原菌在叶际（叶表面）的相互作用的重要作用。该项目的长期目标是阐明导致基于气孔的防御的信号转导途径，并剖析细菌毒素COR抑制这种新型宿主防御的机制。要测试的中心假设是：（i）基于气孔的防御是植物天然免疫反应的组成部分，以限制细菌（包括人类病原菌）的进入，以及（ii）要成功，植物病原体必须依赖于特定的毒力因子或环境条件来抑制基于气孔的防御。一个综合的方法，包括分子遗传学，显微镜，生化和发病机理分析将被使用。拟议的研究将有助于植物先天免疫和细菌致病的基础知识，并将增加我们对植物-人类病原体在叶际相互作用的分子基础的理解。新鲜蔬菜的人类病原体污染是一个主要的公共卫生问题，也是一个潜在的生物恐怖主义威胁，是食物中毒的来源。公共卫生关系：本项目主要研究模式植物拟南芥对植物和人类病原菌天然免疫的分子基础。人类病原体对食品的污染是公众健康的一个主要问题。这项研究有可能提供一个机制的理解植物防御和人类病原体污染的新鲜农产品，并指导未来制定适当的预防措施。